Mouse anti-Human COMMD1 Monoclonal Antibody | anti-COMMD1 antibody

Anti-COMMD1 Antibody (clone 2E2) IHC-plus

Cat. Num. AAA249348

• Gene Names: COMMD1; MURR1; C2orf5
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Protein A/G purified
• Synonyms: COMMD1; Monoclonal Antibody; Anti-COMMD1 Antibody (clone 2E2) IHC-plus; Mouse Monoclonal [clone 2E2] (IgG2a) to Human COMMD1; Human COMMD1; C2orf5; Protein Murr1; Copper metabolism gene MURR1; MURR1; anti-COMMD1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 2E2
• Specificity: Human COMMD1
• Purity/Purification: Protein A/G purified
• Form/Format: PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 190

• Applicable Applications for anti-COMMD1 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Flow Cytometry (1:100); IHC-P (10 ug/ml); WB (1:2000)
• Immunogen: COMMD1 antibody was raised against full length human recombinant protein of human COMMD1(NP_689729) produced in HEK293T cell.
• Immunogen Description: Full length human recombinant protein of human COMMD1(NP_689729) produced in HEK293T cell.
• Immunogen Type: Recombinant protein
• Target Species: Human
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry (IHC)

(Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY COMMD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-COMMD1.)

Western Blot (WB)

(Western blot of extracts (35 ug) from 9 different cell lines by using g anti-COMMD1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).)

Flow Cytometry (FC/FACS)

(Flow cytometry of HeLa cells, using anti-COMMD1 antibody (Red), compared to a nonspecific negative control antibody (Blue).)

Flow Cytometry (FC/FACS)

(HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-COMMD1 antibody, and then analyzed by flow cytometry.)

Flow Cytometry (FC/FACS)

(Flow cytometry of Jurkat cells, using anti-COMMD1 antibody (Red), compared to a nonspecific negative control antibody (Blue).)

Related Product Information for anti-COMMD1 antibody

COMMD1 Antibody, C2orf5 Antibody, Protein Murr1 Antibody, Copper metabolism gene MURR1 Antibody, MURR1 Antibody Description: Promotes ubiquitination of NF-kappa-B subunit RELA and its subsequent proteasomal degradation. Down-regulates NF-kappa-B activity. Down-regulates SOD1 activity by interfering with its homodimerization. Plays a role in copper ion homeostasis. Can bind one copper ion per monomer. May function to facilitate biliary copper excretion within hepatocytes.

NCBI and Uniprot Product Information

• NCBI GI #: 51316026
• NCBI GeneID: 150684
• UniProt Accession #: Q8N668; Q96GS0; B4DFQ4
• Molecular Weight: 17,665 Da
• NCBI Official Full Name: COMM domain-containing protein 1
• NCBI Official Synonym Full Names: copper metabolism domain containing 1
• NCBI Official Symbol: COMMD1
• NCBI Official Synonym Symbols: MURR1; C2orf5
• NCBI Protein Information: COMM domain-containing protein 1
• UniProt Protein Name: COMM domain-containing protein 1
• Protein Family: COMM domain-containing protein
• UniProt Gene Name: COMMD1
• UniProt Synonym Gene Names: C2orf5; MURR1
• UniProt Entry Name: COMD1_HUMAN

NCBI Description

COMMD1 is a regulator of copper homeostasis, sodium uptake, and NF-kappa-B (see MIM 164011) signaling (de Bie et al., 2005 [PubMed 16267171]).[supplied by OMIM, Sep 2009]

Uniprot Description

COMMD1: Promotes ubiquitination of NF-kappa-B subunit RELA and its subsequent proteasomal degradation. Down-regulates NF-kappa-B activity. Down-regulates SOD1 activity by interfering with its homodimerization. Plays a role in copper ion homeostasis. Can bind one copper ion per monomer. May function to facilitate biliary copper excretion within hepatocytes.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 2p15

Cellular Component: Cul2-RING ubiquitin ligase complex; cytoplasm; cytoplasmic membrane-bound vesicle; early endosome; endosome membrane; nucleoplasm; nucleus; recycling endosome

Molecular Function: copper ion binding; identical protein binding; phosphatidylinositol-3,4,5-triphosphate binding; phosphatidylinositol-3,4-bisphosphate binding; phosphatidylinositol-4,5-bisphosphate binding; protein binding; protein homodimerization activity

Biological Process: copper ion homeostasis; Golgi to plasma membrane transport; inhibition of NF-kappaB transcription factor; plasma membrane to endosome transport; positive regulation of protein ubiquitination; protein transport; regulation of proteasomal ubiquitin-dependent protein catabolic process; transcription, DNA-dependent