Human Collagen Type VIII Alpha 1 ELISA Kit | COL8alpha1 elisa kit

Human Collagen Type VIII Alpha 1 ELISA Kit

Cat. Num. AAA763362

• Gene Names: COL8A1; C3orf7
• Reactivity: Human
• Synonyms: Collagen Type VIII Alpha 1; Human Collagen Type VIII Alpha 1 ELISA Kit; COL8alpha1 (Collagen Type VIII Alpha 1); COL8alpha1 elisa kit

• Reactivity: Human
• Sequence Length: 744

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for COL8alpha1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human COL8alpha1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human COL8alpha1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human COL8alpha1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human COL8alpha1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 15488904
• NCBI GeneID: 1295
• Molecular Weight: 73,364 Da
• NCBI Official Full Name: Collagen, type VIII, alpha 1
• NCBI Official Synonym Full Names: collagen type VIII alpha 1
• NCBI Official Symbol: COL8A1
• NCBI Official Synonym Symbols: C3orf7
• NCBI Protein Information: collagen alpha-1(VIII) chain
• UniProt Protein Name: Collagen alpha-1(VIII) chain
• UniProt Gene Name: COL8A1
• UniProt Synonym Gene Names: C3orf7
• UniProt Entry Name: CO8A1_HUMAN

NCBI Description

This gene encodes one of the two alpha chains of type VIII collagen. The gene product is a short chain collagen and a major component of the basement membrane of the corneal endothelium. The type VIII collagen fibril can be either a homo- or a heterotrimer. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Dec 2011]

Uniprot Description

COL8A1: Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in atherogenesis.

Protein type: Secreted, signal peptide; Secreted; Extracellular matrix

Chromosomal Location of Human Ortholog: 3q12.3

Cellular Component: collagen type VIII; endoplasmic reticulum lumen; extracellular matrix; extracellular region; intracellular membrane-bound organelle

Molecular Function: protein binding

Biological Process: angiogenesis; camera-type eye morphogenesis; cell adhesion; collagen catabolic process; epithelial cell proliferation; extracellular matrix disassembly; extracellular matrix organization and biogenesis