Porcine Collagen Type VIII Alpha 1 ELISA Kit | COL8a1 elisa kit

Porcine Collagen Type VIII Alpha 1 ELISA Kit

Cat. Num. AAA736153

• Gene Names: COL8A1; C3orf7
• Reactivity: Porcine
• Synonyms: Collagen Type VIII Alpha 1; Porcine Collagen Type VIII Alpha 1 ELISA Kit; COL8a1 elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This COL8a1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine COL8a1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for COL8a1 elisa kit

Principle of the Assay: COL8a1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-COL8a1 antibody and an COL8a1-HRP conjugate. The assay sample and buffer are incubated together with COL8a1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COL8a1 concentration since COL8a1 from samples and COL8a1-HRP conjugate compete for the anti-COL8a1 antibody binding site. Since the number of sites is limited, as more sites are occupied by COL8a1 from the sample, fewer sites are left to bind COL8a1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COL8a1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for COL8a1 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 15488904
• NCBI GeneID: 1295
• Molecular Weight: 73,364 Da
• NCBI Official Full Name: Collagen, type VIII, alpha 1
• NCBI Official Synonym Full Names: collagen, type VIII, alpha 1
• NCBI Official Symbol: COL8A1
• NCBI Official Synonym Symbols: C3orf7
• NCBI Protein Information: collagen alpha-1(VIII) chain; collagen alpha-1(VIII) chain; smag-64; endothelial collagen; collagen VIII, alpha-1 polypeptide; cell proliferation-inducing protein 41; smooth muscle cell-expressed and macrophage conditioned medium-induced protein smag-64
• UniProt Protein Name: Collagen alpha-1(VIII) chain
• Protein Family: Collagen
• UniProt Gene Name: COL8A1
• UniProt Synonym Gene Names: C3orf7
• UniProt Entry Name: CO8A1_HUMAN

NCBI Description

This gene encodes one of the two alpha chains of type VIII collagen. The gene product is a short chain collagen and a major component of the basement membrane of the corneal endothelium. The type VIII collagen fibril can be either a homo- or a heterotrimer. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Dec 2011]

Uniprot Description

COL8A1: Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in atherogenesis.

Protein type: Extracellular matrix; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3q12.3

Cellular Component: extracellular matrix; intracellular membrane-bound organelle; endoplasmic reticulum lumen; collagen type VIII; extracellular region

Biological Process: extracellular matrix disassembly; collagen catabolic process; epithelial cell proliferation; extracellular matrix organization and biogenesis; camera-type eye morphogenesis; angiogenesis; cell adhesion