COL5A2 recombinant protein

COL5A2 Protein, Human, Recombinant (mFc Tag)

Cat. Num. AAA8121507

• Purity: >85% as determined by SDS-PAGE
• Synonyms: COL5A2; COL5A2 Protein; Human; Recombinant (mFc Tag); Human COL5A2 Protein (Fc Tag); collagen; type V; alpha 2; COL5A2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Leu1229

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 27
• Tag: C-mouse IgG1-Fc
• Protein Construction: A DNA sequence encoding the human COL5A2(P05997)(Met1-Leu1229) was fused with Fc region of mouse IgG at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for COL5A2 recombinant protein

Background: COL5A2 is a component of type V collagen. It is known as the pro-alpha2(V) chain. COL5A2, together with two pro-alpha1(V) chains can form type V procollagen. These triple-stranded, rope-like procollagen molecules arrange themselves into long, thin fibrils that cross-link to one another in the spaces around cells. The cross-links result in the formation of very strong, mature type V collagen fibers. Type V collagen can be detected in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen.

References

Greenspan DS. et al., 1992, Genomics. 12 (4): 836-7. Mann K. 1992, Biol Chem Hoppe-Seyler. 373 (2): 69-75. Greenspan DS. et al., 1992, Gene Expr. 1 (1): 29-39.

NCBI and Uniprot Product Information

• NCBI GI #: 89363017
• NCBI GeneID: 1290
• NCBI Accession #: NP_000384.2
• NCBI GenBank Nucleotide #: NM_000393.3
• UniProt Accession #: P05997; P78440; Q13908; Q53WR4; Q59GR4; Q6LDJ5; Q7KZ55; Q86XF6; Q96QB0; Q96QB3
• Molecular Weight: 144,910 Da
• NCBI Official Full Name: collagen alpha-2(V) chain preproprotein
• NCBI Official Synonym Full Names: collagen, type V, alpha 2
• NCBI Official Symbol: COL5A2
• NCBI Protein Information: collagen alpha-2(V) chain; collagen alpha-2(V) chain; AB collagen; type V preprocollagen alpha 2 chain; collagen, fetal membrane, A polypeptide
• UniProt Protein Name: Collagen alpha-2(V) chain
• Protein Family: Collagen
• UniProt Gene Name: COL5A2
• UniProt Entry Name: CO5A2_HUMAN

NCBI Description

This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. [provided by RefSeq, Jul 2008]

Uniprot Description

COL5A2: Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. Type V collagen is a key determinant in the assembly of tissue- specific matrices. Defects in COL5A2 are a cause of Ehlers-Danlos syndrome type 1 (EDS1); also known as Ehlers-Danlos syndrome gravis or severe classic type Ehlers-Danlos syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome. Defects in COL5A2 are a cause of Ehlers-Danlos syndrome type 2 (EDS2); also known as Ehlers-Danlos syndrome mitis or mild classic type Ehlers Danlos syndrome. Belongs to the fibrillar collagen family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2q14-q32

Cellular Component: extracellular matrix; collagen type V; endoplasmic reticulum lumen; extracellular region

Molecular Function: metal ion binding; extracellular matrix structural constituent; SMAD binding

Biological Process: skin development; collagen catabolic process; extracellular matrix disassembly; axon guidance; extracellular matrix organization and biogenesis; ossification; collagen fibril organization; skeletal development; eye morphogenesis

Disease: Ehlers-danlos Syndrome, Type I