Rabbit anti-Human, mouse Fgfr3 Polyclonal Antibody | anti-Fgfr3 antibody

Mouse Fgfr3 Antibody (Center)

Cat. Num. AAA9212039

• Reactivity: Human, mouse
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: Fgfr3; Polyclonal Antibody; Mouse Fgfr3 Antibody (Center); Fibroblast growth factor receptor 3; FGFR-3; Heparin-binding growth factor receptor; CD333; Mfr3; Sam3; anti-Fgfr3 antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This Mouse Fgfr3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 468-495 amino acids from the Central region of mouse Fgfr3.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 468-495
• Sequence Length: 801

• Applicable Applications for anti-Fgfr3 antibody: ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)
• Application Notes: WB~~1:1000; IHC-P~~1:10~50
• Function: Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation and apoptosis. Plays an essential role in the regulation of chondrocyte differentiation, proliferation and apoptosis, and is required for normal skeleton development. Regulates both osteogenesis and postnatal bone mineralization by osteoblasts. Promotes apoptosis in chondrocytes, but can also promote cancer cell proliferation. Required for normal development of the inner ear. Phosphorylates PLCG1, CBL and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Plays a role in the regulation of vitamin D metabolism. Mutations that lead to constitutive kinase activation or impair normal FGFR3 maturation, internalization and degradation lead to aberrant signaling. Over-expressed or constitutively activated FGFR3 promotes activation of STAT1, STAT5A and STAT5B. Plays a role in postnatal lung development.
• Cellular Location: Cell membrane; Single-pass type I membrane protein. Cytoplasmic vesicle. Endoplasmic reticulum. Note=The activated receptor is rapidly internalized and degraded. Detected in intracellular vesicles after internalization of the autophosphorylated receptor (By similarity).
• Tissue Location: In embryo, expressed in heart, lung, kidney, skin, head and liver but not in muscle. In adult, highest levels in brain Also expressed in liver, lung, kidney, testis, ovary and uterus. Very low levels in heart, thymus, spleen and muscle
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Mouse Fgfr3 Antibody (Center) (MBS9212039) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the Fgfr3 antibody detected the Fgfr3 protein (arrow).)

Immunohistochemistry (IHC)

(Mouse Fgfr3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Fgfr3 Antibody (Center) (MBS9212039M for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-Fgfr3 antibody

Receptor for acidic and basic fibroblast growth factors. Preferentially binds FGF1.

NCBI and Uniprot Product Information

• NCBI GI #: 2497569
• NCBI GeneID: 2497569
• UniProt Accession #: Q61851; Q61564; Q63834
• Molecular Weight: 87758
• NCBI Official Full Name: Fibroblast growth factor receptor 3
• UniProt Protein Name: Fibroblast growth factor receptor 3
• Protein Family: Fibroblast growth factor receptor
• UniProt Gene Name: Fgfr3
• UniProt Synonym Gene Names: Mfr3; Sam3; FGFR-3
• UniProt Entry Name: FGFR3_MOUSE

NCBI Description

This gene encodes a member of the fibroblast growth factor receptor family. Members of this family are highly conserved proteins that differ from one another in their ligand affinities and tissue distribution. A representative protein consists of an extracellular region composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment, and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This family member binds acidic and basic fibroblast growth hormone and plays a role in bone development and maintenance. Mutations in this gene may be associated with craniosynostosis and multiple types of skeletal dysplasia. A pseudogene of this gene is located on chromosome 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Apr 2011]

Uniprot Description

FGFR3: a receptor tyrosine kinase of the highly-conserved FGFR family that binds fibroblast growth factor (FGF). Mutations are associated with thanatophoric dysplasia (TD), craniosynostosis Adelaide type, many craniosynostotic syndromes and bone malformations. Three splice-variant isoforms have been described. Activating point mutations cause dwarfism, including achondroplasia, hypochrondroplasia and thanatophoric dysplasia, and facial and other morphogenetic disorders, including Crouzon syndrome, craniosynostosis Adelaide type, San Diego skeletal displasia and Muenke syndrome. Translocations t(4;14) involving the IgH region are common in multiple myeloma and frequently involve FGFR3. Activated FGFR3 found in 30% of bladder cancers and several cervical cancers, but not in other tumors. Two mutations found in colorectal cancer.

Protein type: EC 2.7.10.1; Kinase, protein; Protein kinase, tyrosine (receptor); Membrane protein, integral; Protein kinase, TK; TK group; FGFR family

Cellular Component: Golgi apparatus; transport vesicle; internal side of plasma membrane; cell surface; membrane; endoplasmic reticulum; integral to plasma membrane; perinuclear region of cytoplasm; lysosome; cytoplasm; integral to membrane; plasma membrane; cytoplasmic vesicle; nucleus

Molecular Function: transferase activity; protein binding; fibroblast growth factor binding; fibroblast growth factor receptor activity; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding; protein kinase activity

Biological Process: peptidyl-tyrosine phosphorylation; somatic stem cell maintenance; apoptosis; protein amino acid autophosphorylation; negative regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; bone mineralization; positive regulation of tyrosine phosphorylation of Stat3 protein; negative regulation of cell proliferation; substantia nigra development; positive regulation of MAPKKK cascade; cell-cell signaling; inner ear receptor cell differentiation; positive regulation of neuron apoptosis; forebrain development; positive regulation of cell proliferation; morphogenesis of an epithelium; response to axon injury; cell differentiation; oligodendrocyte development; skeletal development; inner ear development; negative regulation of epithelial cell proliferation; negative regulation of developmental growth; fibroblast growth factor receptor signaling pathway; myelination in the central nervous system; MAPKKK cascade; positive regulation of phosphoinositide 3-kinase activity; digestive tract morphogenesis; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein ubiquitination; cartilage development; negative regulation of smoothened signaling pathway; negative regulation of mitosis; negative regulation of astrocyte differentiation; lens morphogenesis in camera-type eye; positive regulation of endothelial cell proliferation; positive regulation of cell differentiation; phosphorylation