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Western Blot (WB) (Western Blot showing EphA2 antibody used against NIH/3T3 cell lysate.)

Mouse EPHA2 Monoclonal Antibody | anti-EPHA2 antibody

EPHA2 antibody

Gene Names
EPHA2; ECK; CTPA; ARCC2; CTPP1; CTRCT6
Applications
ELISA, Immunohistochemistry, Western Blot
Synonyms
EPHA2; Monoclonal Antibody; EPHA2 antibody; Monoclonal EPHA2; Anti-EPHA2; EPHA 2; EPHA-2; Ephrin receptor EPHA2; epoxide hydrolase 1 microsomal (xenobiotic); anti-EPHA2 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgM
Clone Number
1B3C7
Form/Format
Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
976
Applicable Applications for anti-EPHA2 antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
ELISA: 1:10,000
IHC: 1:200-1:1000
WB: 1:500-1:2000
Biological Significance
The Eph family of receptors is the largest known subfamily of receptor tyrosine kinases (RTk). Eph RTks and their ligands, the ephrins, are frequently overexpressed in a variety of cancers and tumor cell lines. One Eph family member, EPHA2, is also overexpressed in a wide variety of cancers, including breast, small-cell lung and gastrointestinal cancers, melanomas, and neuroblastomas. The ephrin-Eph interactions are important in development, especially in cell-cell interactions involved in nervous system patterning and possibly in cancer.
Cross-Reactivity
Human
Immunogen
EPHA2 antibody was raised in Mouse using a purified recombinant fragment of EPHA2 expressed in E Coli as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot showing EphA2 antibody used against NIH/3T3 cell lysate.)

Western Blot (WB) (Western Blot showing EphA2 antibody used against NIH/3T3 cell lysate.)
Related Product Information for anti-EPHA2 antibody
Mouse monoclonal EPHA2 antibody
Product Categories/Family for anti-EPHA2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
54,305 Da
NCBI Official Full Name
ephrin type-A receptor 2
NCBI Official Synonym Full Names
EPH receptor A2
NCBI Official Symbol
EPHA2
NCBI Official Synonym Symbols
ECK; CTPA; ARCC2; CTPP1; CTRCT6
NCBI Protein Information
ephrin type-A receptor 2
UniProt Protein Name
Ephrin type-A receptor 2
Protein Family
UniProt Gene Name
EPHA2
UniProt Synonym Gene Names
ECK
UniProt Entry Name
EPHA2_HUMAN

NCBI Description

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders.[provided by RefSeq, May 2010]

Uniprot Description

EphA2: a receptor tyrosine kinase. Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A3, -A4 AND -A5. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. Overexpressed in many cancers including aggressive ovarian, cervical and breast carcinomas, and lung cancer. Expression correlates with degree of angiogenesis, metastasis and xenograft tumor growth. Soluble receptor inhibits tumor growth and angiogenesis in mice.

Protein type: Protein kinase, tyrosine (receptor); Membrane protein, integral; Kinase, protein; Protein kinase, TK; EC 2.7.10.1; TK group; Eph family

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: focal adhesion; cell surface; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; ephrin receptor activity; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: neural tube development; axial mesoderm formation; axon guidance; cell migration; peptidyl-tyrosine phosphorylation; negative regulation of protein kinase B signaling cascade; viral reproduction; multicellular organismal development; notochord formation; mammary gland epithelial cell proliferation; osteoclast differentiation; regulation of blood vessel endothelial cell migration; bone remodeling; regulation of angiogenesis; keratinocyte differentiation; osteoblast differentiation; regulation of cell adhesion mediated by integrin; DNA damage response, signal transduction resulting in induction of apoptosis; notochord cell development; ephrin receptor signaling pathway; protein kinase B signaling cascade; angiogenesis; vasculogenesis; cell adhesion; skeletal development

Disease: Cataract 6, Multiple Types

Research Articles on EPHA2

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Product Notes

The EPHA2 epha2 (Catalog #AAA830498) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's EPHA2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). ELISA: 1:10,000 IHC: 1:200-1:1000 WB: 1:500-1:2000. Researchers should empirically determine the suitability of the EPHA2 epha2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EPHA2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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