Typical Testing Data/Standard Curve (for reference only)
Rat Calponin-1 ELISA Kit | Cnn1 elisa kit
Rat Calponin-1 ELISA Kit
Reactivity
Rat
Synonyms
Calponin-1; Rat Calponin-1 ELISA Kit; Basic calponin; Calponin H1; smooth muscle; Cnn1; Cnn1 elisa kit
Reactivity
Rat
Specificity
This assay recognizes recombinant and natural rat Calponin-1. No significant cross-reactivity or interference was observed.
Sequence Length
297
Assay Type
Sandwich
Detection Range
0.312-20 ng/mL
Sensitivity
0.162 ng/mL
Intra-Assay CV
<=5.8%
Inter-Assay CV
<=8.0%
Recovery
102%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Cnn1 elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of rat Calponin-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calponin-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Calponin-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Calponin-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Calponin-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calponin-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Calponin-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Calponin-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Calponin-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33,344 Da
NCBI Official Full Name
calponin-1
NCBI Official Synonym Full Names
calponin 1
NCBI Official Symbol
Cnn1
NCBI Protein Information
calponin-1
UniProt Protein Name
Calponin-1
Protein Family
UniProt Gene Name
Cnn1
UniProt Entry Name
CNN1_RAT
NCBI Description
binds actin stress fibers; involved in actin bundling [RGD, Feb 2006]
Uniprot Description
calponin 1: Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity. Belongs to the calponin family.
Protein type: Motility/polarity/chemotaxis; Contractile; Myosin-binding; Actin-binding
Cellular Component: cytoskeleton; focal adhesion
Molecular Function: actin binding; calmodulin binding
Biological Process: actomyosin structure organization and biogenesis
Research Articles on Cnn1
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Product Notes
The Rat Cnn1 cnn1 (Catalog #AAA2886964) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2886964 ELISA Kit recognizes Rat Cnn1. It is sometimes possible for the material contained within the vial of "Calponin-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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