Rat Neuropeptide FF receptor 2 ELISA Kit | Npffr2 elisa kit

Rat Neuropeptide FF receptor 2 ELISA Kit

Cat. Num. AAA288420

• Gene Names: Npffr2; Gpr74; Npff2; Npgpr
• Reactivity: Rat
• Synonyms: Neuropeptide FF receptor 2; Rat Neuropeptide FF receptor 2 ELISA Kit; Npffr2 elisa kit

• Reactivity: Rat
• Specificity: This assay recognizes recombinant and natural rat Neuropeptide FF receptor 2. No significant cross-reactivity or interference was observed.
• Sequence Length: 420

• Assay Type: Sandwich
• Detection Range: 0.78-50 ng/ml
• Sensitivity: 0.39 ng/mL
• Intra-Assay CV: <=5.1%
• Inter-Assay CV: <=10.2%
• Recovery: 102%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Npffr2 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuropeptide FF receptor 2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Neuropeptide FF receptor 2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Neuropeptide FF receptor 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Neuropeptide FF receptor 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13027438
• NCBI GeneID: 78964
• NCBI Accession #: NP_076470.1
• NCBI GenBank Nucleotide #: NM_023980.1
• UniProt Accession #: Q9EQD2
• Molecular Weight: 47,711 Da
• NCBI Official Full Name: neuropeptide FF receptor 2
• NCBI Official Synonym Full Names: neuropeptide FF receptor 2
• NCBI Official Symbol: Npffr2
• NCBI Official Synonym Symbols: Gpr74; Npff2; Npgpr
• NCBI Protein Information: neuropeptide FF receptor 2
• UniProt Protein Name: Neuropeptide FF receptor 2
• Protein Family: Neuropeptide FF receptor
• UniProt Gene Name: Npffr2
• UniProt Synonym Gene Names: Gpr74; Npff2; Npgpr
• UniProt Entry Name: NPFF2_RAT

NCBI Description

a receptor for neuropeptide FF (NPFF); may be involved in pain modulation and opiate tolerance [RGD, Feb 2006]

Uniprot Description

NPFFR2: Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol- calcium second messenger system. Belongs to the G-protein coupled receptor 1 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR

Cellular Component: actin cytoskeleton; integral to plasma membrane; plasma membrane

Molecular Function: neuropeptide receptor activity; opioid receptor binding; peptide binding

Biological Process: cellular response to hormone stimulus; neuropeptide signaling pathway; regulation of adenylate cyclase activity; regulation of MAPKKK cascade