CNDP2 recombinant protein

CNDP2, 1-475aa, Human, His tag, E Coli

Cat. Num. AAA204805

• Gene Names: CNDP2; CN2; CPGL; PEPA; HsT2298; HEL-S-13
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: CNDP2; 1-475aa; Human; His tag; E Coli; Cytosolic non-specific dipeptidase; CN2; CPGL; HsT2298; PEPA; CNDP2 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAALTTL FKYIDENQDR YIKKLAKWVA IQSVSAWPEK RGEIRRMMEV AAADVKQLGG SVELVDIGKQ KLPDGSEIPL PPILLGRLGS DPQKKTVCIY GHLDVQPAAL EDGWDSEPFT LVERDGKLYG RGSTDDKGPV AGWINALEAY QKTGQEIPVN VRFCLEGMEE SGSEGLDELI FARKDTFFKD VDYVCISDNY WLGKKKPCIT YGLRGICYFF IEVECSNKDL HSGVYGGSVH EAMTDLILLM GSLVDKRGNI LIPGINEAVA AVTEEEHKLY DDIDFDIEEF AKDVGAQILL HSHKKDILMH RWRYPSLSLH GIEGAFSGSG AKTVIPRKVV GKFSIRLVPN MTPEVVGEQV TSYLTKKFAE LRSPNEFKVY MGHGGKPWVS DFSHPHYLAG RRAMKTVFGV EPDLTREGGS IPVTLTFQEA TGKNVMLLPV GSADDGAHSQ NEKLNRYNYI EGTKMLAAYL YEVSQLKD
• Sequence Length: 475

• Applicable Applications for CNDP2 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for CNDP2 recombinant protein

Cytosolic non-specific dipeptidase, also known as CNDP2, is a cytosolic, non-specific dipeptidase that belongs to the peptidase M20A family of proteins. CNDP2 is a secreted peptidase homologous to M20 peptidases. It is expressed by all adult and fetal tissue, however, an isoform lacking exons 3 and 4 was expressed in all fetal tissue, but only in adult liver. Overexpression of CPGL-B in hepatocellular carcinoma cells leads to significant inhibition of HC cell viability, colony formation, cell invasiveness and tumor formation. Recombinant human CNDP2 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for CNDP2 recombinant protein

Enzymes & Proteases

References

Wanic K., et al. (2008) Diabetes. 57:2547-2551.; Otani H., et al. (2008) Neurosci Lett. 445:166-169.

NCBI and Uniprot Product Information

• NCBI GI #: 271398239
• NCBI GeneID: 55748
• NCBI Accession #: NP_060705
• NCBI GenBank Nucleotide #: NM_018235.2
• UniProt Accession #: Q96KP4; Q8WY59; Q9BQ94; Q9NVB4; B3KUG4; V9HWE5
• Molecular Weight: 55.3kDa (498aa)
• NCBI Official Full Name: cytosolic non-specific dipeptidase isoform 1
• NCBI Official Synonym Full Names: carnosine dipeptidase 2
• NCBI Official Symbol: CNDP2
• NCBI Official Synonym Symbols: CN2; CPGL; PEPA; HsT2298; HEL-S-13
• NCBI Protein Information: cytosolic non-specific dipeptidase
• UniProt Protein Name: Cytosolic non-specific dipeptidase
• Protein Family: Cytosolic non-specific dipeptidase
• UniProt Gene Name: CNDP2

NCBI Description

CNDP2, also known as tissue carnosinase and peptidase A (EC 3.4.13.18), is a nonspecific dipeptidase rather than a selective carnosinase (Teufel et al., 2003 [PubMed 12473676]).[supplied by OMIM, Mar 2008]

Uniprot Description

Hydrolyzes a variety of dipeptides including L-carnosine but has a strong preference for Cys-Gly (PubMed:19346245). Acts as a functional tumor suppressor in gastric cancer via activation of the mitogen-activated protein kinase (MAPK) pathway. An elevated level of CNDP2 activates the p38 and JNK MAPK pathways to induce cell apoptosis, and a lower level of CNDP2 activates the ERK MAPK pathway to promote cell proliferation (PubMed:24395568). Isoform 2 may play a role as tumor suppressor in hepatocellular carcinoma (HCC) cells (PubMed:17121880). Catalyzes the production of N-lactoyl-amino acids from lactate and amino acids by reverse proteolysis (PubMed:25964343).