CLEC14A recombinant protein

CLEC14A Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122671

• Gene Names: Clec14a; AI642649; 1200003C23Rik
• Purity: >95% as determined by SDS-PAGE
• Synonyms: CLEC14A; CLEC14A Protein; Mouse; Recombinant (His Tag); Mouse CLEC14A/EGFR-5 Protein (His Tag); 1200003C23Rik Protein; AI642649 Protein; C-type lectin domain family 14; member A; CLEC14A recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Thr386

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 22
• Tag: C-His
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse CLEC14A (NP_080085.3)(Met 1-Thr 386) was expressed, fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CLEC14A recombinant protein

Background: C-type lectin domain family 14 member A, also known as Epidermal growth factor receptor 5 and CLEC14A, is a member of the C-type lectin domain (CTLD) family that contains one c-type lectin domain and one EGF-like domain. Mouse CLEC14A is a 459 amino acid single-pass type I membrane protein. The superfamily of proteins containing C-type lectin-like domains (CTLDs) is a large group of extracellular Metazoan proteins with diverse functions. The CTLD structure has a characteristic double-loop ('loop-in-a-loop') stabilized by two highly conserved disulfide bridges located at the bases of the loops, as well as a set of conserved hydrophobic and polar interactions. Members of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily share a common fold and are involved in a variety of functions, such as generalized defense mechanisms against foreign agents, discrimination between healthy and pathogen-infected cells, and endocytosis and blood coagulation. Genome-level studies on human, elegans and melanogaster demonstrated almost complete divergence among invertebrate and mammalian families of CTLD-containing proteins (CTLDcps). The vertebrate CTLDcp families were essentially formed early in vertebrate evolution and are completely different from the invertebrate families. The composition of the CTLDcp superfamily in fish and mammals suggests that large scale duplication events played an important role in the evolution of vertebrates.

References

Ebner S, et al. (2003) Evolutionary analysis reveals collective properties and specificity in the C-type lectin and lectin-like domain superfamily. Proteins. 53(1): 44-55.Zelensky AN, et al. (2005) The C-type lectin-like domain superfamily. Gready JE. FEBS J. 272(24): 6179-217.

NCBI and Uniprot Product Information

• NCBI GI #: 31541811
• NCBI GeneID: 66864
• NCBI Accession #: NP_080085.3
• NCBI GenBank Nucleotide #: NM_025809.5
• UniProt Accession #: Q8VCP9; Q3TP72; Q9CXA8; Q9D624; Q9DC55
• Molecular Weight: 49,066 Da
• NCBI Official Full Name: C-type lectin domain family 14 member A
• NCBI Official Synonym Full Names: C-type lectin domain family 14, member a
• NCBI Official Symbol: Clec14a
• NCBI Official Synonym Symbols: AI642649; 1200003C23Rik
• NCBI Protein Information: C-type lectin domain family 14 member A
• UniProt Protein Name: C-type lectin domain family 14 member A
• Protein Family: C-type lectin domain family
• UniProt Gene Name: Clec14a
• UniProt Entry Name: CLC14_MOUSE