Creatine Kinase, Muscle (CKM) Recombinant Protein | CKM recombinant protein

Recombinant Creatine Kinase, Muscle (CKM)

Cat. Num. AAA2012423

• Gene Names: CKM; M-CK
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Creatine Kinase; Muscle (CKM); Recombinant Creatine Kinase; CKM recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-KLNYKPEEEY PDLTKHNNHM AKALTPEIYK KLRDKETPSG FTLDDVIQTGVDNPGHPFIM TVGCVAGDEE SYQVFKDLFD PIIQDRHGGY KPTDKHKTDL NHENLKGGDDLDPNYVLSSR VRTGRSIKGY TLPPHCSRGE RRAVEKLSIE ALNSLTGEFK GKYYPLKSMTEQEQQQLIDD HFLFDKPVSP LLLASGMARD WPDARGIWHN DNKTFLVWVN EEDHLRVISMQKGGNMKEVF RRFCVGLQKI EEIFKKAGHP FMWNEHLGYV LTCPSNLGTG LRGGVHVKLAHLSKHPKFEE ILTRLRLQKR GTGGVDTAAV GSVFDISNAD RLGSSEVEQV QLVVDGVKLMVEMEKKL
• Sequence Length: 381

• Applicable Applications for CKM recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Canis familiaris; Canine (Dog)
• Expression System: Prokaryotic expression
• Residues: Lys11~Leu367 (Accession # P05123) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 545488156
• NCBI GeneID: 476435
• NCBI Accession #: XP_005616506.1
• NCBI GenBank Nucleotide #: XM_005616449.1
• UniProt Accession #: P05123
• Molecular Weight: 42.0kDa
• NCBI Official Full Name: creatine kinase M-type
• NCBI Official Symbol: CKM
• NCBI Official Synonym Symbols: M-CK
• NCBI Protein Information: creatine kinase M-type; creatine kinase, muscle; Creatine kinase M-type; Creatine kinase M chain
• UniProt Protein Name: Creatine kinase M-type
• Protein Family: Creatine kinase
• UniProt Gene Name: CKM
• UniProt Entry Name: KCRM_CANFA

Uniprot Description

Function: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Catalytic activity: ATP + creatine = ADP + phosphocreatine.

Subunit structure: Dimer of identical or non-identical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.

Subcellular location: Cytoplasm.

Sequence similarities: Belongs to the ATP:guanido phosphotransferase family.Contains 1 phosphagen kinase C-terminal domain.Contains 1 phosphagen kinase N-terminal domain.