Rabbit Fos B Polyclonal Antibody | anti-FosB antibody

Fos B (S23) Polyclonal Antibody

Cat. Num. AAA3001447

• Gene Names: FOSB; AP-1; G0S3; GOS3; GOSB
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Fos B; Polyclonal Antibody; Fos B (S23) Polyclonal Antibody; Protein fosB; G0/G1 switch regulatory protein 3; FOSB; G0S3; anti-FosB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: FosB (S23) polyclonal antibody detects endogenous levels of FosB protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 302

• Applicable Applications for anti-FosB antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids N-terminus of Human Fos B.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Fos B (S23) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:sp2/0 whole cell lysateLane3:PC12 whole cell lysate)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of FosB (S23) pAb in paraffin-embedded human prostate carcinoma tissue.)

Related Product Information for anti-FosB antibody

Fos and Jun dimerize to form Activator Protein-1 (AP-1), a transcriptional factor that binds to the 12-O-tetradecanoylphorbol 13-acetate (TPA) response element (TRE) of several cellular and viral genes including human collagenase, metallothionein IIa, stromelysin, interleukin 2, SV40 and polyoma. Fos and Jun contain the leucine-zipper motif that allows for dimerization and an adjacent basic domain required for biological activity. The functionally active form of Fos is in a heterodimer with a member of the Jun family. While Jun family members can form functional homodimers, studies indicate that Fos family members do not self-associate and therefore do not bind DNA on their own. The various dimers differ in their ability to transactivate AP-1 dependent genes.

NCBI and Uniprot Product Information

• NCBI GI #: 166999783
• NCBI GeneID: 2354
• NCBI Accession #: NP_001107643.1
• NCBI GenBank Nucleotide #: NP_001107643.1
• UniProt Accession #: P53539
• Molecular Weight: ~ 35kDa
• NCBI Official Full Name: protein fosB isoform 2
• NCBI Official Synonym Full Names: FosB proto-oncogene, AP-1 transcription factor subunit
• NCBI Official Symbol: FOSB
• NCBI Official Synonym Symbols: AP-1; G0S3; GOS3; GOSB
• NCBI Protein Information: protein fosB
• UniProt Protein Name: Protein fosB
• Protein Family: Protein
• UniProt Gene Name: FOSB
• UniProt Synonym Gene Names: G0S3
• UniProt Entry Name: FOSB_HUMAN

NCBI Description

The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

FosB: FosB interacts with Jun proteins enhancing their DNA binding activity. Heterodimer. Belongs to the bZIP family. Fos subfamily.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: nucleus

Molecular Function: DNA binding; sequence-specific DNA binding; double-stranded DNA binding; transcription factor activity; transcription factor binding

Biological Process: response to drug; transcription from RNA polymerase II promoter; response to cAMP; response to mechanical stimulus; response to morphine; female pregnancy; negative regulation of transcription from RNA polymerase II promoter; response to progesterone stimulus; response to corticosterone stimulus; cellular response to hormone stimulus