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Western Blot (WB)
(Western Blot: Sample: Mouse Brain lysate; Primary Ab: 2ug/ml Rabbit Anti-Canine CKM Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)
Rabbit anti-Dog Creatine Kinase, Muscle (CKM) Polyclonal Antibody | anti-CKM antibody
Polyclonal Antibody to Creatine Kinase, Muscle (CKM)
Gene Names
CKM; M-CK
Reactivity
Dog
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Creatine Kinase; Muscle (CKM); Polyclonal Antibody; Polyclonal Antibody to Creatine Kinase; anti-CKM antibody
Host
Rabbit
Reactivity
Dog
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against CKM. It has been selected for its ability to recognize CKM in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF-KLNYKPEEEY PDLTKHNNHM AKALTPEIYK KLRDKETPSG FTLDDVIQTGVDNPGHPFIM TVGCVAGDEE SYQVFKDLFD PIIQDRHGGY KPTDKHKTDL NHENLKGGDDLDPNYVLSSR VRTGRSIKGY TLPPHCSRGE RRAVEKLSIE ALNSLTGEFK GKYYPLKSMTEQEQQQLIDD HFLFDKPVSP LLLASGMARD WPDARGIWHN DNKTFLVWVN EEDHLRVISMQKGGNMKEVF RRFCVGLQKI EEIFKKAGHP FMWNEHLGYV LTCPSNLGTG LRGGVHVKLAHLSKHPKFEE ILTRLRLQKR GTGGVDTAAV GSVFDISNAD RLGSSEVEQV QLVVDGVKLMVEMEKKL
MGHHHHHHSGSEF-KLNYKPEEEY PDLTKHNNHM AKALTPEIYK KLRDKETPSG FTLDDVIQTGVDNPGHPFIM TVGCVAGDEE SYQVFKDLFD PIIQDRHGGY KPTDKHKTDL NHENLKGGDDLDPNYVLSSR VRTGRSIKGY TLPPHCSRGE RRAVEKLSIE ALNSLTGEFK GKYYPLKSMTEQEQQQLIDD HFLFDKPVSP LLLASGMARD WPDARGIWHN DNKTFLVWVN EEDHLRVISMQKGGNMKEVF RRFCVGLQKI EEIFKKAGHP FMWNEHLGYV LTCPSNLGTG LRGGVHVKLAHLSKHPKFEE ILTRLRLQKR GTGGVDTAAV GSVFDISNAD RLGSSEVEQV QLVVDGVKLMVEMEKKL
Sequence Length
406
Applicable Applications for anti-CKM antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant CKM (Lys11~Leu367) expressed in E.coli.
Cross Reactivity
Dog
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2037886
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Mouse Brain lysate; Primary Ab: 2ug/ml Rabbit Anti-Canine CKM Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)
Western Blot (WB)
(Western Blot: Sample: Mouse Brain lysate; Primary Ab: 2ug/ml Rabbit Anti-Canine CKM Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)
Western Blot (WB)
(Western Blot: Sample: Mouse Heart lysate; Primary Ab: 2ug/ml Rabbit Anti-Canine CKM Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047))
Western Blot (WB)
(Western Blot: Sample: Mouse Heart lysate; Primary Ab: 2ug/ml Rabbit Anti-Canine CKM Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047))
Western Blot (WB)
(Western Blot: Sample: Canine Brain lysate; Primary Ab: 2ug/ml Rabbit Anti-Canine CKM Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047))
Western Blot (WB)
(Western Blot: Sample: Canine Brain lysate; Primary Ab: 2ug/ml Rabbit Anti-Canine CKM Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047))
Western Blot (WB)
(Western Blot: Sample: Canine Heart lysate; Primary Ab: 2ug/ml Rabbit Anti-Canine CKM Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047))
Western Blot (WB)
(Western Blot: Sample: Canine Heart lysate; Primary Ab: 2ug/ml Rabbit Anti-Canine CKM Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047))
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
43,153 Da
NCBI Official Full Name
creatine kinase M-type
NCBI Official Synonym Full Names
creatine kinase, M-type
NCBI Official Symbol
CKM
NCBI Official Synonym Symbols
M-CK
NCBI Protein Information
creatine kinase M-type
UniProt Protein Name
Creatine kinase M-type
Protein Family
UniProt Gene Name
CKM
Uniprot Description
Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Research Articles on CKM
Similar Products
Product Notes
The CKM ckm (Catalog #AAA2002505) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Creatine Kinase, Muscle (CKM) reacts with Dog and may cross-react with other species as described in the data sheet. AAA Biotech's Creatine Kinase, Muscle (CKM) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the CKM ckm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-KLNYKP EEEY PDLTKHNNHM AKALTPEIYK KLRDKETPSG FTLDDVIQTG VDNPGHPFIM TVGCVAGDEE SYQVFKDLFD PIIQDRHGGY KPTDKHKTDL NHENLKGGDD LDPNYVLSSR VRTGRSIKGY TLPPHCSRGE RRAVEKLSIE ALNSLTGEFK GKYYPLKSMT EQEQQQLIDD HFLFDKPVSP LLLASGMARD WPDARGIWHN DNKTFLVWVN EEDHLRVISM QKGGNMKEVF RRFCVGLQKI EEIFKKAGHP FMWNEHLGYV LTCPSNLGTG LRGGVHVKLA HLSKHPKFEE ILTRLRLQKR GTGGVDTAAV GSVFDISNAD RLGSSEVEQV QLVVDGVKLM VEMEKKL. It is sometimes possible for the material contained within the vial of "Creatine Kinase, Muscle (CKM), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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