Testing Data
CD80 protein
CD80-muIg-Purified Preservative Free
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA
Purity
Purified Preservative Free
Synonyms
CD80; CD80-muIg-Purified Preservative Free; Human CD80-muIg Fusion Protein; CD80 protein
Host
CHO cells
Reactivity
Human
Purity/Purification
Purified Preservative Free
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl
Concentration
0.5 mg/ml (varies by lot)
Sequence Length
316
Applicable Applications for CD80 protein
Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes
Blocking Antibody or recombinant protein binding to CD80. Component for ELISA use.
Molecular Structure
A soluble dimeric fusion protein consisting of the extracellular (173aa) domain of human CD80 fused to murine IgG2a Fc + hinge (232 aa). Predicted monomeric molecular weight : 51.3 kd.
Transfectant Cell Line
CHO
Performance
CD80-muIg was reactive in an Enzyme Immuno-assay utilizing a Goat anti-Mouse Ig coated plate for capture, and CD152-muIg/Biotin recombinant protein or anti-CD80/Biotinylated antibody for detection, followed by Streptavidin/HRP and TMB/H2 O2 substrate chromagen.
Production
Human CD80-muIg fusion protein was purified from (low FBS containing) tissue culture supernatant of CHO transfectants.
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl. Product was sterile filtered and vialed under aseptic conditions.
Preparation and Storage
Store at 2 - 5 degree C. Open under aseptic conditions. Freeze/Thawing is not recommended.
Product should retain activity for at least 6 months after shipping date when stored as recommended.
Product should retain activity for at least 6 months after shipping date when stored as recommended.
Testing Data
Testing Data
Related Product Information for CD80 protein
Information: Human CD80 (B7-1) is a costimulatory ligand for CD28 and CTLA-4(1). CD80 is expressed on activated B
cells (2).
References
1. C.B. Thompson, (1995) Cell 81: 979-982. 2. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 682-684.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
CD80 molecule
NCBI Official Synonym Full Names
CD80 molecule
NCBI Official Symbol
CD80
NCBI Protein Information
CD80 molecule; CD80 antigen
Protein Family
Similar Products
Product Notes
The CD80 (Catalog #AAA666797) is a Protein produced from CHO cells and is intended for research purposes only. The product is available for immediate purchase. The CD80-muIg-Purified Preservative Free reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD80 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA). Blocking Antibody or recombinant protein binding to CD80. Component for ELISA use. Researchers should empirically determine the suitability of the CD80 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD80, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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