CD28 protein

CD28-muIg-Purified Preservative Free

Cat. Num. AAA666849

• Reactivity: Human
• Applications: ELISA
• Purity: Purified Preservative Free
• Synonyms: CD28; CD28-muIg-Purified Preservative Free; Human CD28-muIg Fusion Protein (also binds to mouse CD80/CD86); CD28 protein

• Host: CHO cells
• Reactivity: Human
• Purity/Purification: Purified Preservative Free
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 63

• Applicable Applications for CD28 protein: ELISA (EIA)
• Application Notes: Blocking Antibody binding to CD28, Component for ELISA use
• Molecular Structure: A soluble fusion protein consisting of the extracellular (134aa) domain of human CD28 fused to murine IgG2a Fc (233aa).
• Transfectant Cell Line: CHO
• Performance: CD28-muIg was tested for activity and quantitated in EIA. Fusion protein was captured using Goat-anti-mouse antibody coated plate. After 45 minute incubation, plates were washed twice and an excess of Mouse Ig was added to completely block the plate. Anti-CD28/Biotin followed by Streptavidin/HRP, and TMB/H2 O2 were used for detection. Fusion protein was detectable at 4 ng/ml in this assay.
• Production: Fusion protein was Protein A purified from (low FBS containing) tissue culture supernatant of CHO transfectants. Purity was >95% by SDS-PAGE with less than 1% Bovine Immunoglobulin. Product was 0.2 um filtered and vialed under aseptic conditions.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl. Product was sterile filtered and vialed under aseptic conditions.
• Preparation and Storage: Store at 2 - 5 degree C. Open under aseptic conditions. Freeze/Thawing is not recommended.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Related Product Information for CD28 protein

Information: Human CD28 is an important costimulatory molecule found on all CD4+ T cells and on about half of the CD8+T cells. T cell activities attributed to CD28 include, prevention of anergy, induction of cytokine gene transcription, stabilization of cytokine mRNAs and activation of CD8+ cytotoxic T lymphocytes. The ligands for CD28, identified as CD80 (B7-1) and CD86 (B7-2), are immunoglobulin superfamily monomeric transmembrane glycoproteins of 60 kd and 80 kd, respectively.

References

(1.) M.K. Jenkins & J.G. Johnson, (1993) Curr Opin Immunol 5: 361-367. (2.) 61st Forum in Immunology, (1995) Res Immunol 146: 127-205. (3.) I. Kariv, A. Truneh & R.W. Sweet, (1996) J Immunol 157: 29-38.

NCBI and Uniprot Product Information

• NCBI GI #: 192485
• NCBI GeneID: 12487
• Molecular Weight: 25,243 Da
• NCBI Official Full Name: CD28, partial
• NCBI Official Synonym Full Names: CD28 antigen
• NCBI Official Symbol: Cd28
• NCBI Protein Information: T-cell-specific surface glycoprotein CD28
• UniProt Protein Name: T-cell-specific surface glycoprotein CD28
• Protein Family: T-cell-specific surface glycoprotein
• UniProt Gene Name: Cd28
• UniProt Entry Name: CD28_MOUSE

Uniprot Description

CD28: a type I plasma membrane protein Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Binds to B7-1 and -2. Expressed in T cells and plasma cells, but not in less mature B cells. Six splice-variant isoforms have been described.

Protein type: Cell surface; Membrane protein, integral

Cellular Component: membrane; plasma membrane; integral to membrane; immunological synapse; lipid raft; external side of plasma membrane

Molecular Function: protein homodimerization activity; SH3/SH2 adaptor activity; protease binding

Biological Process: positive regulation of isotype switching to IgG isotypes; T cell activation; positive regulation of mitosis; positive regulation of interleukin-2 biosynthetic process; T cell receptor signaling pathway; negative thymic T cell selection; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of inflammatory response to antigenic stimulus; T cell costimulation; immune response; positive regulation of alpha-beta T cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of T cell proliferation; regulatory T cell differentiation