SDS-Page
(SDS-PAGE 4-15% under reducing conditions and visualized by Coomassie blue staining showing a band at approx. 30 kDa)
COVID 19 Spike Glycoprotein S1 RBD Coronavirus Active Protein | COVID-19 active protein
Recombinant Human SARS-CoV-2 Spike Glycoprotein S1 RBD (CHO)
Purity
Affinity chromatography. Purity: >95% by SDS PAGE
Synonyms
COVID 19 Spike Glycoprotein S1 RBD Coronavirus; Recombinant Human SARS-CoV-2 Spike Glycoprotein S1 RBD (CHO); 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike Glycoprotein S1 RBD; Receptor Binding Domain; COVID-19 active protein
Host
CHO Cells
Purity/Purification
Affinity chromatography. Purity: >95% by SDS PAGE
Form/Format
Liquid ; PBS
Concentration
1 mg/ml (varies by lot)
Sequence
Arg319-Phe541
Tag Information
HIS Tag C-Terminus
Extinction Coefficient
33850 M-1.cm-1 Abs 0.1% (=1 g/l) 1.29 assuming all pairs of Cys residues form cystines
Endotoxins
Negative on cell-based assay
Activity
Active form: positive binding with recombinant ACE2 Human. Recognized by CR3022 anti RBD recombinant antibody and Covid-19 positive patients serum samples.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store at -20 degree C. Avoid repeated freeze-thaw cycles Stable one week at 2 to 8 degree C after reconstitution under sterile conditions Stable 12 months -20 degree C to -80 degree C after reconstitution under sterile conditions
SDS-Page
(SDS-PAGE 4-15% under reducing conditions and visualized by Coomassie blue staining showing a band at approx. 30 kDa)
SDS-Page
(SDS-PAGE 4-15% under reducing conditions and visualized by Coomassie blue staining showing a band at approx. 30 kDa)
Western Blot (WB)
(Western Blot: Spike RBD HIS Tag detected with Anti-HIS-Tag antibody HRP conjugate showing a band at approx. 30 kDa)
Western Blot (WB)
(Western Blot: Spike RBD HIS Tag detected with Anti-HIS-Tag antibody HRP conjugate showing a band at approx. 30 kDa)
Related Product Information for COVID-19 active protein
Spike protein (S protein) is one of four structural proteins of Coronavirus (SARS-Cov, SARS-Cov-2, MERS amongst other), S protein plays the most important role in viral attachment, fusion and entry, and it serves as a target for development of antibodies, entry inhibitors and vaccines. In the S protein, the Receptor Binding Domain (RBD) mediates viral entry of SARS-Cov and SARS-Cov-2 C into host cells by its interaction with the membrane receptor ACE2 (angiotensin-converting enzyme 2).
NCBI and Uniprot Product Information
Molecular Weight
Predicted Molecular Weight: 26.1 kDa; SDS Page: Approx. 30 kDa
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Product Notes
The COVID-19 (Catalog #AAA335828) is an Active Protein produced from CHO Cells and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is HIS Tag C-Terminus. The amino acid sequence is listed below: Arg319-Phe 541. It is sometimes possible for the material contained within the vial of "COVID 19 Spike Glycoprotein S1 RBD Coronavirus, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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