Interleukin 12 Active Protein | IL12A active protein

Interleukin 12, Recombinant, Mouse (IL-12)

Cat. Num. AAA650043

• Gene Names: IL12A; P35; CLMF; NFSK; NKSF1; IL-12A
• Purity: Highly Purified; 95% by SDS-PAGE and HPLC analysis. Endotoxin: 0.1ng/ug
• Synonyms: Interleukin 12; Recombinant; Mouse (IL-12); IL12A active protein

• Host: CHO cells
• Purity/Purification: Highly Purified; 95% by SDS-PAGE and HPLC analysis. Endotoxin: 0.1ng/ug
• Form/Format: Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile PBS to 0.1-1mg/ml. Do not vortex. Add protein carrier for long term storage.
• Sequence: p35 Subunit: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SS p40 Subunit: MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS

• Biological Activity: ED50 as determined by the stimulation of IFN-g production by murine splenocytes co-stimulated with IL-18 is <0.1ng/ml, corresponding to a specific activity of 1x10e7.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for IL12A active protein

IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue type mast cells. Among its biological activities IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells and induces the development of IFN-gamma producing Th1 cells. Recombinant murine IL-12 is a 75.0kD heterodimeric glycoprotein consisting of disulfide-linked 35 kDa (p35) and 40kD (p40) subunits (506 total amino acid residues).

Product Categories/Family for IL12A active protein

Growth Factors; Cytokines; Growth Factors-Interleukin

NCBI and Uniprot Product Information

• NCBI GI #: 4323579
• NCBI GeneID: 3592
• UniProt Accession #: P29459; Q96QZ1
• Molecular Weight: 75.0kD
• NCBI Official Full Name: interleukin 12
• NCBI Official Synonym Full Names: interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
• NCBI Official Symbol: IL12A
• NCBI Official Synonym Symbols: P35; CLMF; NFSK; NKSF1; IL-12A
• NCBI Protein Information: interleukin-12 subunit alpha; CLMF p35; IL35 subunit; IL-12 subunit p35; IL-12, subunit p35; interleukin 12, p35; interleukin-12 alpha chain; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; cytotoxic lymphocyte maturation factor 1, p35; cytotoxic lymphocyte maturation factor 35 kDa subunit; natural killer cell stimulatory factor 1, 35 kD subunit
• UniProt Protein Name: Interleukin-12 subunit alpha
• Protein Family: Interleukin
• UniProt Gene Name: IL12A
• UniProt Synonym Gene Names: NKSF1; IL-12A; CLMF p35; NKSF1
• UniProt Entry Name: IL12A_HUMAN

NCBI Description

This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.

Subunit structure: Heterodimer with IL12B; disulfide-linked. The heterodimer is known as interleukin IL-12. Ref.8

Subcellular location: Secreted.

Induction: Down-regulated in response to enterovirus 71 (EV71) infection. Ref.7

Sequence similarities: Belongs to the IL-6 superfamily.

Sequence caution: The sequence AAA59937.1 differs from that shown. Reason: Erroneous initiation.