Typical Testing Data/Standard Curve (for reference only)
Rat Prostaglandin E Synthase 2 ELISA Kit | PTGES2 elisa kit
Rat Prostaglandin E Synthase 2 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: PTGES2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-PTGES2 antibody and an PTGES2-HRP conjugate. The assay sample and buffer are incubated together with PTGES2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PTGES2 concentration since PTGES2 from samples and PTGES2-HRP conjugate compete for the anti-PTGES2 antibody binding site. Since the number of sites is limited, as more sites are occupied by PTGES2 from the sample, fewer sites are left to bind PTGES2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PTGES2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a membrane-associated prostaglandin E synthase, which catalyzes the conversion of prostaglandin H2 to prostaglandin E2. This protein also has been shown to activate the transcription regulated by a gamma-interferon-activated transcription element (GATE). Multiple transcript variants have been found for this gene. [provided by RefSeq, Jun 2009]
Uniprot Description
PTGES2: Isomerase that catalyzes the conversion of unstable intermediate of prostaglandin E2 H2 (PGH2) into the more stable prostaglandin E2 (PGE2) form. May also have transactivation activity toward IFN-gamma (IFNG), possibly via an interaction with CEBPB; however, the relevance of transcription activation activity remains unclear. Belongs to the GST superfamily.
Protein type: Membrane protein, integral; Isomerase; EC 5.3.99.3; Lipid Metabolism - arachidonic acid
Chromosomal Location of Human Ortholog: 9q34.11
Cellular Component: Golgi membrane; mitochondrion; perinuclear region of cytoplasm; integral to membrane; cytosol; nucleus
Molecular Function: electron carrier activity; DNA binding; glutathione binding; lyase activity; heme binding; protein disulfide oxidoreductase activity; prostaglandin-E synthase activity
Biological Process: secretion; cell redox homeostasis; positive regulation of transcription, DNA-dependent; prostaglandin biosynthetic process
Research Articles on PTGES2
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Product Notes
The Rat PTGES2 ptges2 (Catalog #AAA722955) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA722955 ELISA Kit recognizes Rat PTGES2. It is sometimes possible for the material contained within the vial of "Prostaglandin E Synthase 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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