Chicken PLAU/uPA ELISA Kit | PLAU/uPA elisa kit

Chicken PLAU/uPA(Urokinase-Type Plasminogen Activator) ELISA Kit

Cat. Num. AAA2510785

• Gene Names: PLAU; ATF; QPD; UPA; URK; u-PA; BDPLT5
• Reactivity: Chicken
• Synonyms: PLAU/uPA; Chicken PLAU/uPA(Urokinase-Type Plasminogen Activator) ELISA Kit; PLAU/uPA elisa kit

• Reactivity: Chicken
• Specificity: This kit recognizes natural and recombinant Chicken PLAU/uPA. No significant cross-reactivity or interference between Chicken PLAU/uPA and analogues was observed.
• Sequence Length: 375

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 31.25-2000pg/mL
• Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL
• Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Chicken PLAU/uPA concentrations in serum, plasma and other biological fluids.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PLAU/uPA elisa kit

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PLAU/uPA. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PLAU/uPA and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PLAU/uPA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PLAU/uPA. You can calculate the concentration of PLAU/uPA in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 578819560
• NCBI GeneID: 5328
• NCBI Accession #: XP_006717956.1
• NCBI GenBank Nucleotide #: XM_006717893.1
• UniProt Accession #: P00749; Q15844; Q16618; Q53XS3; Q5SWW9; Q969W6; B4DPZ2
• Molecular Weight: 46,908 Da
• NCBI Official Full Name: urokinase-type plasminogen activator isoform X1
• NCBI Official Synonym Full Names: plasminogen activator, urokinase
• NCBI Official Symbol: PLAU
• NCBI Official Synonym Symbols: ATF; QPD; UPA; URK; u-PA; BDPLT5
• NCBI Protein Information: urokinase-type plasminogen activator; U-plasminogen activator; plasminogen activator, urinary
• UniProt Protein Name: Urokinase-type plasminogen activator
• UniProt Gene Name: PLAU
• UniProt Synonym Gene Names: U-plasminogen activator; uPA
• UniProt Entry Name: UROK_HUMAN

NCBI Description

This gene encodes a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. A specific polymorphism in this gene may be associated with late-onset Alzheimer's disease and also with decreased affinity for fibrin-binding. This protein converts plasminogen to plasmin by specific cleavage of an Arg-Val bond in plasminogen. Plasmin in turn cleaves this protein at a Lys-Ile bond to form a two-chain derivative in which a single disulfide bond connects the amino-terminal A-chain to the catalytically active, carboxy-terminal B-chain. This two-chain derivative is also called HMW-uPA (high molecular weight uPA). HMW-uPA can be further processed into LMW-uPA (low molecular weight uPA) by cleavage of chain A into a short chain A (A1) and an amino-terminal fragment. LMW-uPA is proteolytically active but does not bind to the uPA receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]

Uniprot Description

uPA: Specifically cleave the zymogen plasminogen to form the active enzyme plasmin. Defects in PLAU are the cause of Quebec platelet disorder (QPD). QPD is an autosomal dominant bleeding disorder due to a gain-of-function defect in fibrinolysis. Although affected individuals do not exhibit systemic fibrinolysis, they show delayed onset bleeding after challenge, such as surgery. The hallmark of the disorder is markedly increased PLAU levels within platelets, which causes intraplatelet plasmin generation and secondary degradation of alpha-granule proteins. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide; EC 3.4.21.73; Protease

Chromosomal Location of Human Ortholog: 10q22.2

Cellular Component: extracellular space; focal adhesion; cell surface; plasma membrane; extracellular region

Molecular Function: protein binding; serine-type endopeptidase activity

Biological Process: regulation of cell adhesion mediated by integrin; fibrinolysis; regulation of smooth muscle cell migration; smooth muscle cell migration; response to hypoxia; regulation of receptor activity; blood coagulation; proteolysis; chemotaxis; signal transduction; regulation of cell proliferation

Disease: Quebec Platelet Disorder; Alzheimer Disease