Human Urokinase-Type Plasminogen Activator ELISA Kit | PLAU/uPA elisa kit

Human Urokinase-Type Plasminogen Activator ELISA Kit

Cat. Num. AAA764160

• Gene Names: PLAU; ATF; QPD; UPA; URK; u-PA; BDPLT5
• Reactivity: Human
• Synonyms: Urokinase-Type Plasminogen Activator; Human Urokinase-Type Plasminogen Activator ELISA Kit; uPA/PLAU/Urokinase/ATF/plasminogen activator; urokinase/UPA/u-PA/U-plasminogen activator/urinary/urokinase-type plasminogen activator; PLAU/uPA elisa kit

• Reactivity: Human
• Sequence Length: 345

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 62.5-4000pg/ml
• Sensitivity: 37.5pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for PLAU/uPA elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human uPA antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human uPA antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human uPA amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human uPAcan be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 984290529
• NCBI GeneID: 5328
• NCBI Accession #: NP_001306120.1
• NCBI GenBank Nucleotide #: NM_001319191.1
• Molecular Weight: 46,908 Da
• NCBI Official Full Name: urokinase-type plasminogen activator isoform 3
• NCBI Official Synonym Full Names: plasminogen activator, urokinase
• NCBI Official Symbol: PLAU
• NCBI Official Synonym Symbols: ATF; QPD; UPA; URK; u-PA; BDPLT5
• NCBI Protein Information: urokinase-type plasminogen activator
• UniProt Protein Name: Urokinase-type plasminogen activator
• Protein Family: Urokinase-type plasminogen activator
• UniProt Gene Name: PLAU
• UniProt Synonym Gene Names: U-plasminogen activator; uPA
• UniProt Entry Name: UROK_HUMAN

NCBI Description

This gene encodes a secreted serine protease that converts plasminogen to plasmin. The encoded preproprotein is proteolytically processed to generate A and B polypeptide chains. These chains associate via a single disulfide bond to form the catalytically inactive high molecular weight urokinase-type plasminogen activator (HMW-uPA). HMW-uPA can be further processed into the catalytically active low molecular weight urokinase-type plasminogen activator (LMW-uPA). This low molecular weight form does not bind to the urokinase-type plasminogen activator receptor. Mutations in this gene may be associated with Quebec platelet disorder and late-onset Alzheimer's disease. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]

Uniprot Description

uPA: Specifically cleave the zymogen plasminogen to form the active enzyme plasmin. Defects in PLAU are the cause of Quebec platelet disorder (QPD). QPD is an autosomal dominant bleeding disorder due to a gain-of-function defect in fibrinolysis. Although affected individuals do not exhibit systemic fibrinolysis, they show delayed onset bleeding after challenge, such as surgery. The hallmark of the disorder is markedly increased PLAU levels within platelets, which causes intraplatelet plasmin generation and secondary degradation of alpha-granule proteins. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.21.73; Protease; Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10q22.2

Cellular Component: cell surface; extracellular region; extracellular space; focal adhesion; plasma membrane

Molecular Function: protein binding; serine-type endopeptidase activity

Biological Process: angiogenesis; blood coagulation; chemotaxis; embryo implantation; fibrinolysis; positive regulation of cell proliferation; positive regulation of smooth muscle cell migration; proteolysis; regulation of cell adhesion mediated by integrin; regulation of receptor activity; regulation of smooth muscle cell migration; response to activity; response to hyperoxia; signal transduction; skeletal muscle regeneration; smooth muscle cell migration; spermatogenesis

Disease: Alzheimer Disease; Quebec Platelet Disorder