Typical Testing Data/Standard Curve (for reference only)
Chicken FcgammaR II/CD32 ELISA Kit | FcgammaR II/CD32 elisa kit
Chicken FcgammaR II/CD32 (Receptor II for the Fc Fragment of IgG) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to FcgammaR II/CD32. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for FcgammaR II/CD32 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain FcgammaR II/CD32, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of FcgammaR II/CD32. You can calculate the concentration of FcgammaR II/CD32 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
FCGR2B: a transmembrane receptor for the Fc region of complexed or aggregated immunoglobulin gamma (IgG). Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down modulation of previous state of cell activation triggered via antigen receptors on B cells, T cells or via another Fc receptor. Three splice-variant isoforms have been observed.
Protein type: Cell surface; Oncoprotein; Membrane protein, integral
Cellular Component: recycling endosome; cytoskeleton; cell surface; membrane; integral to plasma membrane; cytoplasm; integral to membrane; plasma membrane; extracellular region; external side of plasma membrane; lipid raft
Molecular Function: protein binding; IgG binding; receptor signaling protein activity; immunoglobulin binding
Biological Process: receptor-mediated endocytosis; negative regulation of phagocytosis; negative regulation of interleukin-10 production; negative regulation of immune response; negative regulation of acute inflammatory response to antigenic stimulus; antigen processing and presentation of exogenous peptide antigen via MHC class II; defense response; negative regulation of B cell proliferation; endocytic recycling; mast cell activation; negative regulation of type I hypersensitivity; negative regulation of neutrophil apoptosis; negative regulation of humoral immune response mediated by circulating immunoglobulin; cell surface receptor linked signal transduction; response to bacterium; positive regulation of phagocytosis; negative regulation of immunoglobulin production; immune complex clearance; immune response; negative regulation of inflammatory response to antigenic stimulus; immunoglobulin mediated immune response; phagocytosis, engulfment
Research Articles on FcgammaR II/CD32
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Product Notes
The Chicken FcgammaR II/CD32 fcgr2 (Catalog #AAA2509794) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2509794 ELISA Kit recognizes Chicken FcgammaR II/CD32. It is sometimes possible for the material contained within the vial of "FcgammaR II/CD32, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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