Rat Visfatin (VF) RTU ELISA Kit | VF rtu elisa kit

Rat Visfatin (VF) ELISA Kit

Cat. Num. AAA4503321

• Gene Names: Nampt; Pbef; Pbef1
• Reactivity: Rat
• Synonyms: Visfatin (VF); Rat Visfatin (VF) ELISA Kit; Nampt; NAmPRTase; PBEF; PBEF1; Pre-B-Cell Colony Enhancing Factor; Nicotinamide Phosphoribosyltransferase; VF rtu elisa kit

• Reactivity: Rat
• Sequence Length: 491

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 1.56-100ng/mL
• Sensitivity: 0.55ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VF rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to VF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to VF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain VF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 29293813
• NCBI GeneID: 297508
• NCBI Accession #: NP_808789.1
• NCBI GenBank Nucleotide #: NM_177928.3
• UniProt Accession #: Q80Z29; Q2VT47
• Molecular Weight: 55,438 Da
• NCBI Official Full Name: nicotinamide phosphoribosyltransferase
• NCBI Official Synonym Full Names: nicotinamide phosphoribosyltransferase
• NCBI Official Symbol: Nampt
• NCBI Official Synonym Symbols: Pbef; Pbef1
• NCBI Protein Information: nicotinamide phosphoribosyltransferase
• UniProt Protein Name: Nicotinamide phosphoribosyltransferase
• UniProt Gene Name: Nampt
• UniProt Synonym Gene Names: Pbef1; NAmPRTase; Nampt; PBEF

NCBI Description

has similarity to cytokines but is not secreted; localized to the nucleus and cytoplasm, changes in subcellular localization may be associated with the cell cycle [RGD, Feb 2006]

Uniprot Description

Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-ARNTL/BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression.