Mouse Catalase ELISA Kit | Cat elisa kit

Mouse Catalase ELISA Kit

Cat. Num. AAA761439

• Reactivity: Mouse
• Synonyms: Catalase; Mouse Catalase ELISA Kit; Cat/Cas1/CAT/Cs-1/catalase/EC 1.11.1.6/MGC138422/MGC138424; Cat elisa kit

• Reactivity: Mouse
• Sequence Length: 527

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.6-1000pg/ml
• Sensitivity: 9.375pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Cat elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Cat antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Cat antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Cat amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Cat can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4557014
• NCBI GeneID: 847
• NCBI Accession #: NP_001743.1
• NCBI GenBank Nucleotide #: NM_001752.3
• Molecular Weight: 59,756 Da
• NCBI Official Full Name: catalase
• NCBI Official Synonym Full Names: catalase
• NCBI Official Symbol: CAT
• NCBI Protein Information: catalase
• UniProt Protein Name: Catalase
• Protein Family: Catalase
• UniProt Gene Name: CAT
• UniProt Entry Name: CATA_HUMAN

NCBI Description

This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

Catalase: Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. Homotetramer. Belongs to the catalase family.

Protein type: Apoptosis; Hydrolase; Mitochondrial; Energy Metabolism - methane; Endoplasmic reticulum; EC 1.11.1.6; Amino Acid Metabolism - tryptophan; Oxidoreductase

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: cytosol; endoplasmic reticulum; extracellular space; focal adhesion; Golgi apparatus; intracellular membrane-bound organelle; lysosome; membrane; mitochondrial intermembrane space; mitochondrion; peroxisomal matrix; peroxisomal membrane; peroxisome; plasma membrane

Molecular Function: aminoacylase activity; antioxidant activity; catalase activity; enzyme binding; heme binding; metal ion binding; NADP binding; oxidoreductase activity, acting on peroxide as acceptor; protein homodimerization activity; receptor binding

Biological Process: activation of NF-kappaB transcription factor; aerobic respiration; aging; cholesterol metabolic process; hemoglobin metabolic process; hydrogen peroxide catabolic process; inhibition of NF-kappaB transcription factor; negative regulation of apoptosis; nucleobase, nucleoside and nucleotide metabolic process; osteoblast differentiation; positive regulation of cell division; positive regulation of phosphoinositide 3-kinase cascade; protein homotetramerization; protein tetramerization; purine base metabolic process; purine nucleotide catabolic process; response to activity; response to cadmium ion; response to drug; response to estradiol stimulus; response to ethanol; response to hydrogen peroxide; response to hyperoxia; response to hypoxia; response to inactivity; response to insulin stimulus; response to L-ascorbic acid; response to lead ion; response to light intensity; response to ozone; response to reactive oxygen species; response to vitamin A; response to vitamin E; triacylglycerol metabolic process; ureteric bud development; UV protection

Disease: Acatalasemia