Catalase (CAT) Assay Kit | CAT assay kit

Catalase (CAT) assay kit (Visible light method)

Cat. Num. AAA2540413

• Synonyms: Catalase (CAT); Catalase (CAT) assay kit (Visible light method); CAT assay kit

• Sequence Length: 527

• Samples: Animal serum, plasma and tissue homogenate samples
• Detection Range: 0.27-155.4 U/mL
• Sensitivity: 0.27 U/mL
• Intra-assay Precision: The intra-assay CV is 3.1%.
• Inter-assay Precision: The inter-assay CV is 5. 1%.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for CAT assay kit

Principle of the Assay: The reaction that catalase (CAT) decomposes H2O2 can be quickly stopped by ammonium molybdate. The residual H2O2 reacts with ammonium molybdate to generate a yellowish complex. CAT activity can be calculated by production of the yellowish complex at 405 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 4557014
• NCBI GeneID: 847
• NCBI GenBank Nucleotide #: NM_001752.3
• Molecular Weight: 59,756 Da
• NCBI Official Full Name: catalase
• NCBI Official Synonym Full Names: catalase
• NCBI Official Symbol: CAT
• NCBI Protein Information: catalase
• UniProt Protein Name: Catalase
• UniProt Gene Name: CAT
• UniProt Entry Name: CATA_HUMAN

NCBI Description

This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

Catalase: Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. Homotetramer. Belongs to the catalase family.

Protein type: Amino Acid Metabolism - tryptophan; Apoptosis; EC 1.11.1.6; Endoplasmic reticulum; Energy Metabolism - methane; Hydrolase; Mitochondrial; Oxidoreductase

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: focal adhesion; intracellular membrane-bound organelle; membrane; mitochondrion; peroxisomal matrix; peroxisomal membrane; peroxisome

Molecular Function: antioxidant activity; catalase activity; enzyme binding; heme binding; NADP binding; oxidoreductase activity, acting on peroxide as acceptor; protein homodimerization activity; receptor binding

Biological Process: hydrogen peroxide catabolic process; negative regulation of apoptosis; osteoblast differentiation; protein homotetramerization; protein tetramerization; purine nucleotide catabolic process; response to hydrogen peroxide; response to reactive oxygen species; UV protection

Disease: Acatalasemia