Rabbit Catalase (CAT) ELISA Kit | CAT elisa kit

Rabbit Catalase (CAT) ELISA Kit

Cat. Num. AAA2600401

• Reactivity: Rabbit
• Synonyms: Catalase (CAT); Rabbit Catalase (CAT) ELISA Kit; CAT elisa kit

• Reactivity: Rabbit
• Specificity: No cross-reaction with other factors.
• Sequence Length: 527

• Samples: Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant CAT concentration
• Assay Type: Sandwich
• Detection Range: 20 ng/ml-0.312 ng/ml
• Sensitivity: 0.06 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CAT elisa kit

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat CAT monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 4557014
• NCBI GeneID: 847
• NCBI Accession #: NP_001743.1
• NCBI GenBank Nucleotide #: NM_001752.3
• Molecular Weight: 59,756 Da
• NCBI Official Full Name: catalase
• NCBI Official Synonym Full Names: catalase
• NCBI Official Symbol: CAT
• NCBI Protein Information: catalase
• UniProt Protein Name: Catalase
• UniProt Gene Name: CAT
• UniProt Entry Name: CATA_HUMAN

NCBI Description

This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

Catalase: Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. Homotetramer. Belongs to the catalase family.

Protein type: Endoplasmic reticulum; Apoptosis; EC 1.11.1.6; Mitochondrial; Hydrolase; Oxidoreductase; Energy Metabolism - methane; Amino Acid Metabolism - tryptophan

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: Golgi apparatus; peroxisomal membrane; peroxisomal matrix; focal adhesion; intracellular membrane-bound organelle; membrane; lysosome; endoplasmic reticulum; plasma membrane; mitochondrial intermembrane space; peroxisome; cytosol

Molecular Function: antioxidant activity; oxidoreductase activity, acting on peroxide as acceptor; protein homodimerization activity; enzyme binding; catalase activity; metal ion binding; heme binding; NADP binding; receptor binding; aminoacylase activity

Biological Process: cholesterol metabolic process; nucleobase, nucleoside and nucleotide metabolic process; hemoglobin metabolic process; purine base metabolic process; activation of NF-kappaB transcription factor; protein homotetramerization; positive regulation of phosphoinositide 3-kinase cascade; osteoblast differentiation; response to reactive oxygen species; response to vitamin E; response to hyperoxia; triacylglycerol metabolic process; UV protection; hydrogen peroxide catabolic process; ureteric bud development; inhibition of NF-kappaB transcription factor; positive regulation of cell division; aerobic respiration; response to hypoxia; purine nucleotide catabolic process; protein tetramerization; negative regulation of apoptosis; aging

Disease: Acatalasemia