Complement Factor B (CFB) Recombinant Protein | CFB recombinant protein

Recombinant Complement Factor B (CFB)

Cat. Num. AAA2011759

• Gene Names: Cfb; B; Bf; C2; Fb; H2-Bf; AI195813; AI255840
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Complement Factor B (CFB); Recombinant Complement Factor B (CFB); CFB recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in 10mM PBS, pH 7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and preservative.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-LCGMVWEHKK GNDYHKQPWQ AKISVTRPLK GHETCMGAVV SEYFVLTAAH CFMVDDQKHS IKVSVGGQRR DLEIEEVLFH PKYNINGKKA EGIPEFYDYD VALVKLKNKL KYGQTLR
• Sequence Length: 761

• Applicable Applications for CFB recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Leu474~Arg590 (Accession # P04186) with N-terminal His-Tag
• Predicted Molecular Mass: 15 kDa
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile ddH2O
• Predicted Isoelectric Point: 8.7
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 116126
• NCBI GeneID: 14962
• UniProt Accession #: P04186
• Molecular Weight: 15.0kDa
• NCBI Official Full Name: Complement factor B
• NCBI Official Synonym Full Names: complement factor B
• NCBI Official Symbol: Cfb
• NCBI Official Synonym Symbols: B; Bf; C2; Fb; H2-Bf; AI195813; AI255840
• NCBI Protein Information: complement factor B; Factor B; properdin; C3/C5 convertase; complement component 2 (within H-2S); alternative-complement pathway C3/C5 convertase; histocompatibility 2, complement component factor B
• UniProt Protein Name: Complement factor B
• Protein Family: Complement factor
• UniProt Gene Name: Cfb
• UniProt Synonym Gene Names: Bf; H2-Bf
• UniProt Entry Name: CFAB_MOUSE

Uniprot Description

CFB: Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B- lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes. Defects in CFB are a cause of susceptibility to hemolytic uremic syndrome atypical type 4 (AHUS4). An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; EC 3.4.21.47; Protease; Secreted

Cellular Component: extracellular space; extracellular region

Molecular Function: peptidase activity; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; catalytic activity

Biological Process: cell proliferation; immune system process; complement activation, alternative pathway; innate immune response; proteolysis; complement activation