Human factor B (BF) ELISA Kit | BF elisa kit

Human factor B (BF) ELISA Kit

Cat. Num. AAA744362

• Gene Names: Cfb; B; Bf; C2; Fb; H2-Bf; AI195813; AI255840
• Reactivity: Human
• Synonyms: factor B (BF); Human factor B (BF) ELISA Kit; BF elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BF elisa kit

Principle of the Assay: BF ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BF antibody and an BF-HRP conjugate. The assay sample and buffer are ncubated together with BF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells arethen incubated with a substrate for HRP enzyme. The product of the enzyme-substratereaction forms a blue colored complex. Finally, a stop solution is added to stop thereaction, which will then turn the solution yellow. The intensity of color is measuredspectrophotometrically at 450nm in a microplate reader. The intensity of the color isinversely proportional to the BF concentration since BF from samples and BF-HRPconjugate compete for the anti-BF antibody binding site. Since the number of sites islimited, as more sites are occupied by BF from the sample, fewer sites are left to bindBF-HRP conjugate. A standard curve is plotted relating the intensity of the color(O.D.) to the concentration of standards. The BF concentration in each sample isinterpolated from this standard curve.

Intended Uses: This BF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human BF. This ELISA kit for research use only!

NCBI and Uniprot Product Information

• NCBI GI #: 192414
• NCBI GeneID: 14962
• UniProt Accession #: P04186
• Molecular Weight: 85,004 Da
• NCBI Official Full Name: factor B
• NCBI Official Synonym Full Names: complement factor B
• NCBI Official Symbol: Cfb
• NCBI Official Synonym Symbols: B; Bf; C2; Fb; H2-Bf; AI195813; AI255840
• NCBI Protein Information: complement factor B; Factor B; properdin; C3/C5 convertase; complement component 2 (within H-2S); alternative-complement pathway C3/C5 convertase; histocompatibility 2, complement component factor B
• UniProt Protein Name: Complement factor B
• UniProt Gene Name: Cfb
• UniProt Synonym Gene Names: Bf; H2-Bf
• UniProt Entry Name: CFAB_MOUSE

Uniprot Description

CFB: Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B- lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes. Defects in CFB are a cause of susceptibility to hemolytic uremic syndrome atypical type 4 (AHUS4). An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Protease; EC 3.4.21.47

Cellular Component: extracellular space; extracellular region

Molecular Function: peptidase activity; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; catalytic activity

Biological Process: cell proliferation; immune system process; complement activation, alternative pathway; innate immune response; proteolysis; complement activation