Carboxylesterase 1G Recombinant Protein | CES1G recombinant protein

Recombinant Mouse Carboxylesterase 1G

Cat. Num. AAA146795

• Gene Names: Ces1g; Ces1; Ces-1; Ses-1
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: Carboxylesterase 1G; Recombinant Mouse Carboxylesterase 1G; CES1G Mouse; Liver carboxylesterase 1; Acyl-coenzyme A: cholesterol acyltransferase; ES-x; CES1G; Carboxylesterase 1G Mouse Recombinant; CES1G recombinant protein

• Host: Sf9, Baculovirus cells
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: Sterile filtered colorless solution.; CES1G protein solution (0.5mg/ml) containing Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
• Sequence: ADPHPSLPPVVHTVHGKVLG KYVTLEGFSQ PVAVFLGVPF AKPPLGSLRF APPEPAEPWS FVKHTTSYPP LCYQNPEAALRLAELFTNQR KIIPHKFSED CLYLNIYTPA DLTQNSRLPV MVWIHGGGLV IDGASTYDGV PLAVHENVVVVVIQYRLGIW GFFSTEDEHS RGNWGHLDQV AALHWVQDNI ANFGGNPGSV TIFGESAGGE SVSVLVLSPLAKNLFHRAIA QSSVIFNPCL FGRAARPLAK KIAALAGCKT TTSAAMVHCL RQKTEDELLE VSLKMKFGTVDFLGDPRESY PFLPTVIDGV LLPKAPEEIL AEKSFNTVPY MVGINKHEFG WIIPMFLDFP LSERKLDQKT AASILWQAYPILNISEKLIP AAIEKYLGGT EDPATMTDLF LDLIGDIMFG VPSVIVSRSH RDAGAPTYMY EYQYRPSFVSDDRPQELLGD HADELFSVWG APFLKEGASE EEINLSKMVM KFWANFARNG NPNGEGLPHW PEYDQKEGYLQIGVPAQAAH RLKDKEVDFW TELRAKETAE RSSHREHVEL HHHHHH
• Sequence Length: 565

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Related Product Information for CES1G recombinant protein

Description: CES1G produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 556 amino acids (19-565 a.a.) and having a molecular mass of 61.9kDa (Migrates at 50-70kDa on SDS-PAGE under reducing conditions). CES1G is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Introduction: Carboxylesterase 1 (CES1G) belongs to a large family of carboxylesterases that are liable for the hydrolysis of ester and amide bonds. CES1G is also participates in the detoxification of xenobiotics prodrugs. CES1G shares the serine hydrolase fold observed in other esterases. CES1G found in rats and mice and is expressed mainly in liver, but also in kidney and lung.

Product Categories/Family for CES1G recombinant protein

Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 162287349
• NCBI GeneID: 12623
• NCBI Accession #: NP_067431.2
• NCBI GenBank Nucleotide #: NM_021456.4
• UniProt Accession #: Q8VCC2; O55136
• Molecular Weight: 62,680 Da
• NCBI Official Full Name: liver carboxylesterase 1
• NCBI Official Synonym Full Names: carboxylesterase 1G
• NCBI Official Symbol: Ces1g
• NCBI Official Synonym Symbols: Ces1; Ces-1; Ses-1
• NCBI Protein Information: liver carboxylesterase 1
• UniProt Protein Name: Liver carboxylesterase 1
• UniProt Gene Name: Ces1
• UniProt Synonym Gene Names: Ces1g

NCBI Description

This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This enzyme is the major liver enzyme and functions in liver drug clearance. The expression and activity of this gene is age-related but independent of growth hormone level. This gene is clustered with Ces7 and Ces3 on chromosome 8. [provided by RefSeq, Jun 2010]

Uniprot Description

CES1: Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate. Belongs to the type-B carboxylesterase/lipase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.1.1; EC 3.1.1.56; Hydrolase; Xenobiotic Metabolism - drug metabolism - other enzymes

Chromosomal Location of Human Ortholog: 8 C5|8 45.29 cM

Cellular Component: extracellular space

Biological Process: regulation of gene expression