Testing Data
(Fig-1: Induction of STAT1 activity by IFN-gamma in STAT1 Leeporter HeLa cells.)
STAT1 cell line
STAT1 Leeporter HeLa Cell Line
Testing Data
(Fig-1: Induction of STAT1 activity by IFN-gamma in STAT1 Leeporter HeLa cells.)
Testing Data
(Fig-1: Induction of STAT1 activity by IFN-gamma in STAT1 Leeporter HeLa cells.)
NCBI and Uniprot Product Information
Uniprot Description
STAT1: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of certain receptor tyrosine kinases, GPCRs, and receptors for various interleukins and interferons. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 1 C1.1|1 26.81 cM
Cellular Component: axon; cell-cell adherens junction; cytoplasm; dendrite; nuclear chromatin; nucleolus; nucleoplasm; nucleus; perinuclear region of cytoplasm
Molecular Function: CCR5 chemokine receptor binding; DNA binding; double-stranded DNA binding; enzyme binding; nuclear hormone receptor binding; protein homodimerization activity; protein phosphatase 2A binding; sequence-specific DNA binding; transcription factor activity; tumor necrosis factor receptor binding
Biological Process: blood circulation; caspase activation; cellular response to insulin stimulus; cytokine and chemokine mediated signaling pathway; JAK-STAT cascade; lipopolysaccharide-mediated signaling pathway; negative regulation of angiogenesis; negative regulation of endothelial cell proliferation; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of viral protein levels in host cell; positive regulation of cell proliferation; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of smooth muscle cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; response to bacterium; response to cAMP; response to cytokine stimulus; response to drug; response to exogenous dsRNA; response to lipopolysaccharide; response to mechanical stimulus; response to nutrient; response to peptide hormone stimulus; transcription, DNA-dependent; tumor necrosis factor-mediated signaling pathway
Research Articles on STAT1
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Product Notes
The STAT1 stat1 (Catalog #AAA668877) is a Cell Line and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's STAT1 can be used in a range of immunoassay formats including, but not limited to, Functional Assay. Monitor the STAT1 signaling pathway activity. Screen for activators or inhibitors of the STAT1 signaling pathway. Culture conditions: Cells should be grown at 37 degree C with 5% CO2 using DMEM medium supplemented with 10% FBS and 1% Pen/Strep, plus 3 ug/ml of Puromycin. It is recommended to quickly thaw the frozen cells upon receipt or from liquid nitrogen in a 37 degree C water-bath, transfer to a tube containing 10 ml of growth medium without Puromycin, spin down cells, resuspend cells in pre-warmed growth medium without Puromycin, transfer resuspended cells to T25 flask and culture in 37 degree C-CO2 incubator. Leave the T25 flask in the incubator for 2~4 days without disturbing or changing the medium until cells completely recover viability and become adherent. Once cells are over 90% adherent, remove growth medium and passage the cells through trypsinization and centrifugation. At first passage, switch to growth medium containing Puromycin. Cells should be split before they reach complete confluence. To passage the cells, detach cells from culture vessel with Trypsin/EDTA, add complete growth medium and transfer to a tube, spin down cells, resuspend cells and seed appropriate aliquots of cells suspension into new culture vessels. Subcultivation ration = 1: 10 to 1: 20 weekly. Functional validation: A. Response of STAT1 Leeporter- HeLa cells to IFN-gamma. 1. Harvest STAT1 Leeporter- HeLa cells and seed cells into a white solid-bottom 96-well microplate in 100 ul of growth medium at 5 x 10^4 cells/well. 2. Incubate cells at 37 degree C in a CO2 incubator for overnight. 3. The next day, stimulate cells with different concentrations of human IFN-gamma. 4. Incubate at 37 degree C in a CO2 incubator for 6-16 hours. 5. Add 30-50 ul of luciferase assay reagent per well. 6. Incubate at room temperature for 1-5 minutes and measure luminescence using a microplate luminometer. Researchers should empirically determine the suitability of the STAT1 stat1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STAT1, Cell Line" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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