Testing Data
(Fig-1: Induction of NF-kB activity by phorbol 12-myristate 13-acetate (PMA) in NF-kB Leeporter HEK293 cells.)
NF-Kb cell line
NF-kB Leeporter HEK293 Cell Line
Testing Data
(Fig-1: Induction of NF-kB activity by phorbol 12-myristate 13-acetate (PMA) in NF-kB Leeporter HEK293 cells.)
Testing Data
(Fig-1: Induction of NF-kB activity by phorbol 12-myristate 13-acetate (PMA) in NF-kB Leeporter HEK293 cells.)
NCBI and Uniprot Product Information
NCBI Description
gene encodes a 105 kD protein that can be processed to create a 50 kD protein; the larger protein is a Rel protein-specific transcription inhibitor and the smaller protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex [RGD, Feb 2006]
Uniprot Description
NFkB-p105: a transcription factor of the nuclear factor-kappaB ( NFkB) group. Undergoes cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of NFkB. NFkB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFkB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFkB has been associated with a number of inflammatory diseases while persistent inhibition of NFkB leads to inappropriate immune cell development or delayed cell growth. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Two alternatively spliced isoforms have been described.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 2q43
Cellular Component: cytoplasm; cytosol; mitochondrion; neuron projection; nucleoplasm; nucleus; protein complex
Molecular Function: actinin binding; chromatin binding; DNA binding; double-stranded DNA binding; heat shock protein binding; identical protein binding; protein binding; protein heterodimerization activity; protein homodimerization activity; sequence-specific DNA binding; transcription factor activity; transcription factor binding
Biological Process: I-kappaB kinase/NF-kappaB cascade; inflammatory response; innate immune response; negative regulation of apoptosis; negative regulation of cytokine production; negative regulation of inflammatory response; negative regulation of interleukin-12 biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; response to bacterium; response to copper ion; response to organic cyclic substance; response to oxidative stress
Research Articles on NF-Kb
Similar Products
Product Notes
The NF-Kb nfkb1 (Catalog #AAA668866) is a Cell Line and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's NF-Kb can be used in a range of immunoassay formats including, but not limited to, Functional Assay. Monitor the NF-kB signaling pathway. Screen for activators or inhibitors of the NF-kB signaling pathway. Culture conditions: Cells should be grown at 37 degree C with 5% CO2 using DMEM medium supplemented with 10% FBS and 1% Pen/Strep, plus 3 ug/ml of Puromycin. It is recommended to quickly thaw the frozen cells upon receipt or from liquid nitrogen in a 37 degree C water-bath, transfer to a tube containing 10 ml of growth medium without Puromycin, spin down cells, resuspend cells in pre-warmed growth medium without Puromycin, transfer resuspended cells to T25 flask and culture in 37 degree C-CO2 incubator. Leave the T25 flask in the incubator for 2~4 days without disturbing or changing the medium until cells completely recover viability and become adherent. Once cells are over 90% adherent, remove growth medium and passage the cells through trypsinization and centrifugation. At first passage, switch to growth medium containing Puromycin. Cells should be split before they reach complete confluence. To passage the cells, detach cells from culture vessel with Trypsin/EDTA, add complete growth medium and transfer to a tube, spin down cells, resuspend cells and seed appropriate aliquots of cells suspension into new culture vessels. Subcultivation ration = 1: 10 to 1: 20 weekly. Functional validation: A. Response of NF-kB Leeporter- HEK293 cells to phorbol 12-myristate 13-acetate (PMA). 1. Harvest NF-kB Leeporter- HEK293 cells and seed cells into a white solid-bottom 96-well microplate in 100 ul of growth medium at 5 x 10^4 cells/well. 2. Incubate cells at 37 degree C in a CO2 incubator for overnight. 3. The next day, stimulate cells with different concentrations of PMA. 4. Incubate at 37 degree C in a CO2 incubator for 6-16 hours. 5. Add 30-50 ul of luciferase assay reagent per well. 6. Incubate at room temperature for 1-5 minutes and measure luminescence using a microplate luminometer. Researchers should empirically determine the suitability of the NF-Kb nfkb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NF-Kb, Cell Line" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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