Typical Testing Data/Standard Curve (for reference only)
Rabbit SDC4 ELISA Kit | SDC4 elisa kit
Rabbit SDC4 (Syndecan 4) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SDC4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for SDC4and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SDC4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of SDC4.You can calculate the concentration of SDC4in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. The encoded protein is found as a homodimer and is a member of the syndecan proteoglycan family. This gene is found on chromosome 20, while a pseudogene has been found on chromosome 22. [provided by RefSeq, Jul 2008]
Uniprot Description
syndecan-4: a heparan sulfate proteoglycan type I membrane protein that belongs to the syndecan proteoglycan family. Expressed as a homodimer. Promotes cell spreading in a beta(1) integrin-dependent fashion through PKC-alpha and Rho.
Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 20q12
Cellular Component: lysosomal lumen; cell surface; focal adhesion; costamere; integral to plasma membrane; Golgi lumen; plasma membrane; lipid raft
Molecular Function: protein binding; protein kinase C binding; fibronectin binding
Biological Process: phototransduction, visible light; extracellular matrix organization and biogenesis; glycosaminoglycan metabolic process; pathogenesis; chondroitin sulfate metabolic process; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; positive regulation of focal adhesion formation; ureteric bud development; positive regulation of protein kinase activity; carbohydrate metabolic process; positive regulation of stress fiber formation; retinoid metabolic process
Research Articles on SDC4
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Product Notes
The Rabbit SDC4 sdc4 (Catalog #AAA2512609) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2512609 ELISA Kit recognizes Rabbit SDC4. It is sometimes possible for the material contained within the vial of "SDC4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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