Testing Data
Mouse Monocyte/Macrophage Antibody
Mouse Anti-Chicken Monocyte/Macrophage-BIOT
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunocytochemistry
Synonyms
Monocyte/Macrophage; Mouse Anti-Chicken Monocyte/Macrophage-BIOT; anti-Monocyte/Macrophage antibody
Host
Mouse
Isotype
IgG1kappa
Clone Number
KUL01
Specificity
Chicken Monocyte/Macrophage
Concentration
0.5 mg/mL (varies by lot)
Applicable Applications for anti-Monocyte/Macrophage antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Formalin/ Paraffin, Immunocytochemistry (ICC)
Application Notes
Flow Cytometry - Quality tested; Immunohistochemistry-Frozen Sections - Reported in literature; Immunohistochemistry-Paraffin Sections - Reported in literature; Immunocytochemistry - Reported in literature
Immunogen
Chicken peripheral blood mononuclear cell leukocytes
Buffer Formulation
Supplied in PBS/NaN3
Isotype Control
Mouse IgG1-BIOT
Preparation and Storage
Store at 2-8 degree C
Testing Data
Testing Data
Related Product Information for anti-Monocyte/Macrophage antibody
The monoclonal antibody KUL01 is useful in the study of the development, distribution, function, and ontogeny of the mononuclear phagocyte system (MPS) of the chicken by exclusively recognizing the cells of the MPS. It identifies chicken monocytes and macrophages as well as interdigitating cells and activated microglia cells. This antibody does not react with B (Bu-1+) or T (CD3+) lymphocytes.
References
1. Mast J, Goddeeris BM, Peeters K, Vandesande F, Berghman LR. Characterisation of chicken monocytes, macrophages and interdigitating cells by the monoclonal antibody KUL01. Vet Immunol Immunopathol. 1998;61:343-57. (Immunogen, IHC-FS, FC)
2. Khatri M, Palmquist JM, Cha RM, Sharma JM. Infection and activation of bursal macrophages by virulent infectious bursal disease virus. Virus Res. 2005;113:44-50. (FC, ICC)
3. Nagy N, Bíró É, Takács Á, Pólos M, Magyar A, Oláh I. Peripheral blood fibrocytes contribute to the formation of the avian spleen. Dev Dyn. 2005;232:55-66. (IHC-FS)
4. Igyártó B, Lackó E, Oláh I, Magyar A. Characterization of chicken epidermal dendritic cells. Immunology. 2006;119:278-88. (IHC-FS)
5. Abdul-Careem MK, Hunter DB, Thanthrige-Don N, Haghighi HR, Lambourne MD, Sharif S. Cellular and cytokine responses associated with dinitrofluorobenzene-induced contact hypersensitivity in the chicken. Vet Immunol Immunopathol. 2008;122:275-84. (IHC-FS)
6. Abdul-Careem MK, Hunter BD, Sarson AJ, Parvizi P, Haghighi HR, Read L, et al. Host responses are induced in feathers of chickens infected with Marek's disease virus. Virology. 2008;370:323-32. (IHC-FS)
7. van Haarlam DA, van Kooten PJ, Rothwell L, Kaiser P, Vervelde L. Characterisation and expression analysis of the chicken interleukin-7 receptor alpha chain. Dev Comp Immunol. 2009;33:1018-26. (FC, IHC-FS)
8. Bader SR, Kothlow S, Trapp S, Schwarz SC, Philipp H, Weigend S, et al. Acute paretic syndrome in juvenile White Leghorn chickens resembles late stages of acute inflammatory demyelinating polyneuropathies in humans. J Neuroinflammation. 2010;7:7. (FC, IHC-FS)
9. Levkut M, Revajová V, Lauková A, Šev?íková Z, Spišáková V, Faixová Z, et al. Leukocytic responses and intestinal mucin dynamics of broilers protected with Enterococcus faecium O49EF55 and challenged with Salmonella Enteritidis. Res Vet Sci. 2012;93:195-201. (IHC-FS)
10. Warchol ME, Schwendener RA, Hirose K. Depletion of resident macrophages does not alter sensory regeneration in the avian cochlea. PLoS One. 2012;7(12):e51574. (IHC-FS)
11. Pielsticker C. Investigations on the humoral and cell-mediated immune response in chicken after inoculation with Campylobacter jejuni strains of human and avian origin [thesis]. Hannover (Germany): University of Veterinary Medicine Hannover; 2013. (IHC-FS)
12. Kameka AM, Haddadi S, Jamaldeen FJ, Moinul P, He XT, Nawazdeen FH, et al. Clodronate treatment significantly depletes macrophages in chickens. Can J Vet Res. 2014;78:274-82. (FC, IHC-FS)
13. Teixeira AR, Gomes C, Nitz N, Sousa AO, Alves RM, Guimaro MC, et al. Trypanosoma cruzi in the chicken model: Chagas-like heart disease in the absence of parasitism. PLoS Negl Trop Dis. 2011;5(3):e1000. (IHC-PS)
14. Rebel JM, Peeters B, Fijten H, Post J, Cornelissen J, Vervelde L. Highly pathogenic or low pathogenic avian influenza virus subtype H7N1 infection in chicken lungs: small differences in general acute responses. Vet Res. 2011;42:10. (IHC-PS)
15. Reemers SS, van Haarlem D, Groot Koerkamp MJ, Vervelde L. Differential gene-expression and host-response profiles against avian influenza virus within the chicken lung due to anatomy and airflow. J Gen Virol. 2009;90:2134-46. (IHC-FS)
16. Reemers SS, Jansen C, Koerkamp MJ, van Haarlem D, van de Haar P, Degen WG, et al. Reduced immune reaction prevents immunopathology after challenge with avian influenza virus: a transcriptomics analysis of adjuvanted vaccines. Vaccine. 2010;28:6351-60. (IHC-FS)
17. Viertlboeck BC, Schweinsberg S, Hanczaruk MA, Schmitt R, Du Pasquier L, Herberg FW, et al. The chicken leukocyte receptor complex encodes a primordial, activating, high-affinity IgY Fc receptor. Proc Natl Acad Sci USA. 2007;104:11718-23. (FC)
18. Del Cacho E, Gallego M, Lillehoj HS, López-Bernard F, Sánchez-Acedo C. Avian follicular and interdigitating dendritic cells: isolation and morphologic, phenotypic, and functional analyses. Vet Immunol Immunopathol. 2009;129:66-75. (FC)
19. Janardhana V, Broadway MM, Bruce MP, Lowenthal JW, Geier MS, Hughes RJ, et al. Prebiotics modulate immune responses in the gut-associated lymphoid tissue of chickens. J Nutr. 2009;139:1404-9. (FC)
20. Matulova M, Stepanova H, Sisak F, Havlickova H, Faldynova M, Kyrova K, et al. Cytokine signaling in splenic leukocytes from vaccinated and non-vaccinated chickens after intravenous infection with Salmonella enteritidis. PLoS One. 2012;7(2):e32346. (FC)
21. Fernandes Filho T, Fávaro C Jr, Ingberman M, Beiro BC, Inoue A, Gomes L, et al. Effect of spray Escherichia coli vaccine on the immunity of poultry. Avian Dis. 2013;57:671-6. (FC)
22. Vu Manh T, Marty H, Sibille P, Le Vern Y, Kaspers B, Dalod M, et al. Existence of conventional dendritic cells in Gallus gallus revealed by comparative gene expression profiling. J Immunol. 2014;192:4510-7. (FC)
23. Machado PC Jr, Beiro BC, Filho TF, Lourenço MC, Joineau ML, Santin E, et al. Use of blends of organic acids and oregano extracts in feed and water of broiler chickens to control Salmonella Enteritidis persistence in the crop and ceca of experimentally infected birds. J Appl Poult Res. 2014;23:671-82. (FC)
2. Khatri M, Palmquist JM, Cha RM, Sharma JM. Infection and activation of bursal macrophages by virulent infectious bursal disease virus. Virus Res. 2005;113:44-50. (FC, ICC)
3. Nagy N, Bíró É, Takács Á, Pólos M, Magyar A, Oláh I. Peripheral blood fibrocytes contribute to the formation of the avian spleen. Dev Dyn. 2005;232:55-66. (IHC-FS)
4. Igyártó B, Lackó E, Oláh I, Magyar A. Characterization of chicken epidermal dendritic cells. Immunology. 2006;119:278-88. (IHC-FS)
5. Abdul-Careem MK, Hunter DB, Thanthrige-Don N, Haghighi HR, Lambourne MD, Sharif S. Cellular and cytokine responses associated with dinitrofluorobenzene-induced contact hypersensitivity in the chicken. Vet Immunol Immunopathol. 2008;122:275-84. (IHC-FS)
6. Abdul-Careem MK, Hunter BD, Sarson AJ, Parvizi P, Haghighi HR, Read L, et al. Host responses are induced in feathers of chickens infected with Marek's disease virus. Virology. 2008;370:323-32. (IHC-FS)
7. van Haarlam DA, van Kooten PJ, Rothwell L, Kaiser P, Vervelde L. Characterisation and expression analysis of the chicken interleukin-7 receptor alpha chain. Dev Comp Immunol. 2009;33:1018-26. (FC, IHC-FS)
8. Bader SR, Kothlow S, Trapp S, Schwarz SC, Philipp H, Weigend S, et al. Acute paretic syndrome in juvenile White Leghorn chickens resembles late stages of acute inflammatory demyelinating polyneuropathies in humans. J Neuroinflammation. 2010;7:7. (FC, IHC-FS)
9. Levkut M, Revajová V, Lauková A, Šev?íková Z, Spišáková V, Faixová Z, et al. Leukocytic responses and intestinal mucin dynamics of broilers protected with Enterococcus faecium O49EF55 and challenged with Salmonella Enteritidis. Res Vet Sci. 2012;93:195-201. (IHC-FS)
10. Warchol ME, Schwendener RA, Hirose K. Depletion of resident macrophages does not alter sensory regeneration in the avian cochlea. PLoS One. 2012;7(12):e51574. (IHC-FS)
11. Pielsticker C. Investigations on the humoral and cell-mediated immune response in chicken after inoculation with Campylobacter jejuni strains of human and avian origin [thesis]. Hannover (Germany): University of Veterinary Medicine Hannover; 2013. (IHC-FS)
12. Kameka AM, Haddadi S, Jamaldeen FJ, Moinul P, He XT, Nawazdeen FH, et al. Clodronate treatment significantly depletes macrophages in chickens. Can J Vet Res. 2014;78:274-82. (FC, IHC-FS)
13. Teixeira AR, Gomes C, Nitz N, Sousa AO, Alves RM, Guimaro MC, et al. Trypanosoma cruzi in the chicken model: Chagas-like heart disease in the absence of parasitism. PLoS Negl Trop Dis. 2011;5(3):e1000. (IHC-PS)
14. Rebel JM, Peeters B, Fijten H, Post J, Cornelissen J, Vervelde L. Highly pathogenic or low pathogenic avian influenza virus subtype H7N1 infection in chicken lungs: small differences in general acute responses. Vet Res. 2011;42:10. (IHC-PS)
15. Reemers SS, van Haarlem D, Groot Koerkamp MJ, Vervelde L. Differential gene-expression and host-response profiles against avian influenza virus within the chicken lung due to anatomy and airflow. J Gen Virol. 2009;90:2134-46. (IHC-FS)
16. Reemers SS, Jansen C, Koerkamp MJ, van Haarlem D, van de Haar P, Degen WG, et al. Reduced immune reaction prevents immunopathology after challenge with avian influenza virus: a transcriptomics analysis of adjuvanted vaccines. Vaccine. 2010;28:6351-60. (IHC-FS)
17. Viertlboeck BC, Schweinsberg S, Hanczaruk MA, Schmitt R, Du Pasquier L, Herberg FW, et al. The chicken leukocyte receptor complex encodes a primordial, activating, high-affinity IgY Fc receptor. Proc Natl Acad Sci USA. 2007;104:11718-23. (FC)
18. Del Cacho E, Gallego M, Lillehoj HS, López-Bernard F, Sánchez-Acedo C. Avian follicular and interdigitating dendritic cells: isolation and morphologic, phenotypic, and functional analyses. Vet Immunol Immunopathol. 2009;129:66-75. (FC)
19. Janardhana V, Broadway MM, Bruce MP, Lowenthal JW, Geier MS, Hughes RJ, et al. Prebiotics modulate immune responses in the gut-associated lymphoid tissue of chickens. J Nutr. 2009;139:1404-9. (FC)
20. Matulova M, Stepanova H, Sisak F, Havlickova H, Faldynova M, Kyrova K, et al. Cytokine signaling in splenic leukocytes from vaccinated and non-vaccinated chickens after intravenous infection with Salmonella enteritidis. PLoS One. 2012;7(2):e32346. (FC)
21. Fernandes Filho T, Fávaro C Jr, Ingberman M, Beiro BC, Inoue A, Gomes L, et al. Effect of spray Escherichia coli vaccine on the immunity of poultry. Avian Dis. 2013;57:671-6. (FC)
22. Vu Manh T, Marty H, Sibille P, Le Vern Y, Kaspers B, Dalod M, et al. Existence of conventional dendritic cells in Gallus gallus revealed by comparative gene expression profiling. J Immunol. 2014;192:4510-7. (FC)
23. Machado PC Jr, Beiro BC, Filho TF, Lourenço MC, Joineau ML, Santin E, et al. Use of blends of organic acids and oregano extracts in feed and water of broiler chickens to control Salmonella Enteritidis persistence in the crop and ceca of experimentally infected birds. J Appl Poult Res. 2014;23:671-82. (FC)
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Product Notes
The Monocyte/Macrophage (Catalog #AAA670606) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Monocyte/Macrophage can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Formalin/ Paraffin, Immunocytochemistry (ICC). Flow Cytometry - Quality tested; Immunohistochemistry-Frozen Sections - Reported in literature; Immunohistochemistry-Paraffin Sections - Reported in literature; Immunocytochemistry - Reported in literature. Researchers should empirically determine the suitability of the Monocyte/Macrophage for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Monocyte/Macrophage, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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