CELA3A recombinant protein

Recombinant Human CELA3A Protein

Cat. Num. AAA8248775

• Gene Names: CELA3A; ELA3; ELA3A
• Reactivity: Human
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Synonyms: CELA3A; Recombinant Human CELA3A Protein; ELA3; ELA3A; Chymotrypsin-like elastase family member 3A; Elastase IIIA; Elastase-3A; Protease E; CELA3A recombinant protein

• Host: Human cells
• Reactivity: Human
• Purity/Purification: Greater than 95% as determined by reducing SDS-PAGE.
• Form/Format: Supplied as a 0.2 muM filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 7.5.
• Sequence: SGYGPPSSHS SSRVVHGEDA VPYSWPWQVS LQYEKSGSFY HTCGGSLIAP DWVVTAGHCI SRDLTYQVVL GEYNLAVKEG PEQVIPINSE ELFVHPLWNR SCVACGNDIA LIKLSRSAQL GDAVQLASLP PAGDILPNKT PCYITGWGRL YTNGPLPDKL QQARLPVVDY KHCSRWNWWG STVKKTMVCA GGYIRSGCNG DSGGPLNCPT EDGGWQVHGV TSFVSGFGCN FIWKPTVFTR VSAFIDWIEE TIASHVDHHH HHH
• Sequence Length: 270

• Applicable Applications for CELA3A recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. It is recommended that the protein be aliquotted for optimal storage. Avoid repeated feeze-thaw cycles.
• Preparation and Storage: Store it at -20°C to -80°C for one year.

Related Product Information for CELA3A recombinant protein

Recombinant Human CELA3A Protein is produced by our mammalian expression system and the target gene encoding Ser16-His270 is expressed with a 6His tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 236460050
• NCBI GeneID: 10136
• NCBI Accession #: NP_005738.4
• NCBI GenBank Nucleotide #: NM_005747.4
• UniProt Accession #: P09093; Q9BRW4; B1AQ53
• Molecular Weight: 29,489 Da
• NCBI Official Full Name: chymotrypsin-like elastase family member 3A preproprotein
• NCBI Official Synonym Full Names: chymotrypsin like elastase family member 3A
• NCBI Official Symbol: CELA3A
• NCBI Official Synonym Symbols: ELA3; ELA3A
• NCBI Protein Information: chymotrypsin-like elastase family member 3A
• UniProt Protein Name: Chymotrypsin-like elastase family member 3A
• Protein Family: Chymotrypsin-like elastase family
• UniProt Gene Name: CELA3A
• UniProt Synonym Gene Names: ELA3; ELA3A
• UniProt Entry Name: CEL3A_HUMAN

NCBI Description

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3A has little elastolytic activity. Like most of the human elastases, elastase 3A is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3A preferentially cleaves proteins after alanine residues. Elastase 3A may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1. [provided by RefSeq, Jul 2008]

Uniprot Description

CELA3A: Efficient protease with alanine specificity but only little elastolytic activity. Belongs to the peptidase S1 family. Elastase subfamily.

Protein type: EC 3.4.21.70; Protease

Chromosomal Location of Human Ortholog: 1p36.12

Cellular Component: extracellular space

Biological Process: cholesterol metabolic process; digestion