Western Blot (WB)
(Anti-SLC19A1 Picoband antibody, MBS178107, Western blottingAll lanes: Anti SLC19A1 (MBS178107) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: U937 Whole Cell Lysate at 40ugLane 3: SW620 Whole Cell Lysate at 40ugPredicted bind size: 65KDObserved bind size: 65KD )
anti-Human SLC19A1 Polyclonal Antibody | anti-SLC19A1 antibody
Anti-SLC19A1 Antibody
Gene Names
SLC19A1; CHMD; FOLT; IFC1; REFC; RFC1
Reactivity
Human
Applications
Western Blot
Purity
Immunogen Affinity Purified
Synonyms
SLC19A1; Polyclonal Antibody; Anti-SLC19A1 Antibody; Folate transporter 1; CHMD; FLOT 1; FLOT1; FOLT; IFC 1; IFC-1; IFC1; Intestinal folate carrier 1; Intestinal folate carrier; OTTHUMP00000115459; OTTHUMP00000115460; Placental folate transporter; Reduced folate carrier; Reduced folate carrier protein; REFC; RFC 1; RFC; RFC1; S19A1_HUMAN; Solute carrier family 19 member 1; solute carrier family 19 (folate transporter); member 1; anti-SLC19A1 antibody
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
489
Applicable Applications for anti-SLC19A1 antibody
Western Blot (WB)
Application Notes
Western Blot Concentration: 0.1-0.5ug/ml
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human SLC19A1 (202-231aa FLKRPKRSLFFNRDDRGRCETSASELERMN).
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Anti-SLC19A1 Picoband antibody, MBS178107, Western blottingAll lanes: Anti SLC19A1 (MBS178107) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: U937 Whole Cell Lysate at 40ugLane 3: SW620 Whole Cell Lysate at 40ugPredicted bind size: 65KDObserved bind size: 65KD )
Western Blot (WB)
(Anti-SLC19A1 Picoband antibody, MBS178107, Western blottingAll lanes: Anti SLC19A1 (MBS178107) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: U937 Whole Cell Lysate at 40ugLane 3: SW620 Whole Cell Lysate at 40ugPredicted bind size: 65KDObserved bind size: 65KD )
Related Product Information for anti-SLC19A1 antibody
Description: Rabbit IgG polyclonal antibody for Folate transporter 1(SLC19A1) detection. Tested with WB in Human.
Background: Solute carrier family 19 (folate transporter), member 1, also known as SLC19A1 or RFC1, is a protein which in humans is encoded by the SLC19A1 gene. Transport of folate compounds into mammalian cells can occur via receptor-mediated or carrier-mediated mechanisms. A functional coordination between these 2 mechanisms has been proposed to be the method of folate uptake in certain cell types. Methotrexate (MTX) is an antifolate chemotherapeutic agent that is actively transported by the carrier-mediated uptake system. And RFC1 plays a role in maintaining intracellular concentrations of folate.
Background: Solute carrier family 19 (folate transporter), member 1, also known as SLC19A1 or RFC1, is a protein which in humans is encoded by the SLC19A1 gene. Transport of folate compounds into mammalian cells can occur via receptor-mediated or carrier-mediated mechanisms. A functional coordination between these 2 mechanisms has been proposed to be the method of folate uptake in certain cell types. Methotrexate (MTX) is an antifolate chemotherapeutic agent that is actively transported by the carrier-mediated uptake system. And RFC1 plays a role in maintaining intracellular concentrations of folate.
References
1. "Entrez Gene: SLC19A1 solute carrier family 19 (folate transporter), member 1". 2. Lapenta V, Sossi V, Gosset P, Vayssettes C, Vitali T, Rabatel N, Tassone F, Blouin JL, Scott HS, Antonarakis SE, Créau N, Brahe C (April 1998). "Construction of a 2.5-Mb integrated physical and gene map of distal 21q22.3". Genomics 49 (1): 1-13. 3. Stanisawska-Sachadyn A, Mitchell LE, Woodside JV, Buckley PT, Kealey C, Young IS, Scott JM, Murray L, Boreham CA, McNulty H, Strain JJ, Whitehead AS (September 2009). "The reduced folate carrier (SLC19A1) c.80G>A polymorphism is associated with red cell folate concentrations among women". Ann. Hum. Genet. 73 (Pt 5): 484-491.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54,262 Da
NCBI Official Full Name
folate transporter 1 isoform 2
NCBI Official Synonym Full Names
solute carrier family 19 member 1
NCBI Official Symbol
SLC19A1
NCBI Official Synonym Symbols
CHMD; FOLT; IFC1; REFC; RFC1
NCBI Protein Information
folate transporter 1
UniProt Protein Name
Folate transporter 1
UniProt Gene Name
SLC19A1
UniProt Synonym Gene Names
FLOT1; RFC1; FOLT; IFC-1; RFC
UniProt Entry Name
S19A1_HUMAN
NCBI Description
The membrane protein encoded by this gene is a transporter of folate and is involved in the regulation of intracellular concentrations of folate. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
Uniprot Description
SLC19A1: a transporter protein. Transports reduced folate, antitumor agents and folate antagonists such as methotrexate. Apparently involved in methotrexate resistance in various cancers and neural tube defects.
Protein type: Transporter, SLC family; Membrane protein, multi-pass; Transporter; Membrane protein, integral
Chromosomal Location of Human Ortholog: 21q22.3
Cellular Component: apical plasma membrane; basolateral plasma membrane; brush border membrane; integral to plasma membrane; plasma membrane
Molecular Function: folic acid binding; folic acid transporter activity; methotrexate transporter activity; reduced folate carrier activity
Biological Process: female pregnancy; folic acid metabolic process; folic acid transport; methotrexate transport
Research Articles on SLC19A1
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Product Notes
The SLC19A1 slc19a1 (Catalog #AAA178107) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-SLC19A1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SLC19A1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot Concentration: 0.1-0.5ug/ml. Researchers should empirically determine the suitability of the SLC19A1 slc19a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SLC19A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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