UBE2N cdna clone

UBE2N

Cat. Num. AAA200559

• Gene Names: UBE2N; UBC13; UbcH13; HEL-S-71; UbcH-ben; UBCHBEN; UBC13
• Synonyms: UBE2N; HEL-S-71; UBC13; UbcH-ben; UbcH13; UBCHBEN; UBE2N cdna clone

• Form/Format: Lyophilized
• Sequence: Nucleotide Sequence: ATGGCCGGGC TGCCCCGCAG GATCATCAAG GAAACCCAGC GTTTGCTGGC AGAACCAGTT CCTGGCATCA AAGCCGAACC AGATGAGAGC AACGCCCGTT ATTTTCATGT GGTCATTGCT GGCCCTCAGG ATTCCCCCTT TGAGGGAGGG ACTTTTAAAC TTGAACTATT CCTTCCAGAA GAATACCCAA TGGCAGCCCC TAAAGTACGT TTCATGACCA AAATTTATCA TCCTAATGTA GACAAGTTGG GAAGAATATG TTTAGATATT TTGAAAGATA AGTGGTCCCC AGCACTGCAG ATCCGCACAG TTCTGCTATC GATCCAGGCC TTGTTAAGTG CTCCCAATCC AGATGATCCA TTAGCAAATG ATGTAGCGGA GCAGTGGAAG ACCAACGAAG CCCAAGCCAT AGAAACAGCT AGAGCATGGA CTAGGCTATA TGCCATGAAT AATATTTAA; ; Translation Sequence: MAGLPRRIIK ETQRLLAEPV PGIKAEPDES NARYFHVVIA GPQDSPFEGG TFKLELFLPE EYPMAAPKVR FMTKIYHPNV DKLGRICLDI LKDKWSPALQ IRTVLLSIQA LLSAPNPDDP LANDVAEQWK TNEAQAIETA RAWTRLYAMN NI
• Sequence Length: 152

• Species: Human
• Chromosome Location: 12q22
• OMIM Reference Number: 603679
• cDNA Size: 459bp
• Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.
• Vector: (puc19-derived cloning vector)
• Preparation Before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid.
• Preparation and Storage: Store the plasmid at -20 degree C.

Related Product Information for UBE2N cdna clone

uBE2N, also known as uBC13, is a member of the E2 ubiquitin-conjugating enzyme family. It catalyzes the ATP-dependent synthesis of non-canonical polyubiquitin chains, a process that does not lead to proteasomal degradation. It mediates the transcription of several target genes and is thought to play a role in cell cycle progression; cellular differentiation and DNA repair mechanisms that ensure cell survival after DNA damage.

Product Categories/Family for UBE2N cdna clone

Ubiquitination

NCBI and Uniprot Product Information

• NCBI GI #: 4507793
• NCBI GeneID: 7334
• NCBI Accession #: NP_003339.1
• NCBI GenBank Nucleotide #: NM_003348.3
• UniProt Accession #: P61088
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 N
• NCBI Official Synonym Full Names: ubiquitin conjugating enzyme E2 N
• NCBI Official Symbol: UBE2N
• NCBI Official Synonym Symbols: UBC13; UbcH13; HEL-S-71; UbcH-ben; UBCHBEN; UBC13
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 N
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 N
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2N
• UniProt Synonym Gene Names: BLU
• UniProt Entry Name: UBE2N_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair. [provided by RefSeq, Jul 2008]

Uniprot Description

UBE2N: The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Heterodimer with UBE2V2. Interacts (UBE2V2-UBE2N heterodimer) with the E3 ligase STUB1 (via the U-box domain); the complex has a specific 'Lys-63'-linked polyubiquitination activity. Interacts with RNF8 and RNF168. Interacts with RNF11. Interacts with the E3 ligases, HLTF and SHPRH; the interactions promote the 'Lys-63'-linked polyubiquitination of PCNA upon genotoxic stress and lead to DNA repair. Interacts with ARIH2 (via RING-type 2). Belongs to the ubiquitin-conjugating enzyme family.

Protein type: Ligase; Ubiquitin conjugating system; EC 6.3.2.19; Ubiquitin ligase; DNA repair, damage

Chromosomal Location of Human Ortholog: 12q22

Cellular Component: protein complex; cytoplasm; nucleus; cytosol; ubiquitin ligase complex; UBC13-MMS2 complex

Molecular Function: ubiquitin binding; protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; regulation of histone ubiquitination; positive regulation of DNA repair; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of histone modification; postreplication repair; cytokine and chemokine mediated signaling pathway; DNA double-strand break processing; protein modification process; protein ubiquitination; proteolysis; T cell receptor signaling pathway; double-strand break repair via homologous recombination; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; regulation of DNA repair; toll-like receptor 5 signaling pathway; positive regulation of ubiquitin-protein ligase activity; histone ubiquitination; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway