RPA3 cdna clone

RPA3

Cat. Num. AAA200610

• Gene Names: RPA3; REPA3; RP-A p14
• Synonyms: RPA3; REPA3; RPA3 cdna clone

• Form/Format: Lyophilized
• Sequence: Nucleotide Sequence: ATGGTGGACA TGATGGACTT GCCCAGGTCG CGCATCAACG CCGGCATGCT AGCTCAATTC ATCGACAAGC CTGTCTGCTT CGTAGGGAGG CTGGAAAAGA TTCATCCCAC CGGAAAAATG TTTATTCTTT CAGATGGAGA AGGAAAAAAT GGAACCATCG AGTTGATGGA ACCCCTTGAT GAAGAAATCT CTGGAATTGT GGAAGTGGTT GGAAGAGTAA CCGCCAAGGC CACCATCTTG TGTACATCTT ATGTCCAGTT TAAAGAAGAT AGCCATCCTT TTGATCTTGG ACTTTACAAT GAAGCTGTGA AAATTATCCA TGACTTCCCT CAGTTTTATC CTTTAGGGAT TGTGCAACAT GATTGA; ; Translation Sequence: MVDMMDLPRS RINAGMLAQF IDKPVCFVGR LEKIHPTGKM FILSDGEGKN GTIELMEPLD EEISGIVEVV GRVTAKATIL CTSYVQFKED SHPFDLGLYN EAVKIIHDFP QFYPLGIVQH D
• Sequence Length: 121

• Species: Human
• Chromosome Location: 7p22
• OMIM Reference Number: 179837
• cDNA Size: 366bp
• Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.
• Vector: (puc19-derived cloning vector)
• Preparation Before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid.
• Preparation and Storage: Store the plasmid at -20 degree C.

Related Product Information for RPA3 cdna clone

Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

Product Categories/Family for RPA3 cdna clone

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 4506587
• NCBI GeneID: 6119
• NCBI Accession #: NP_002938.1
• NCBI GenBank Nucleotide #: NM_002947.3
• UniProt Accession #: P35244
• NCBI Official Full Name: replication protein A 14 kDa subunit
• NCBI Official Synonym Full Names: replication protein A3
• NCBI Official Symbol: RPA3
• NCBI Official Synonym Symbols: REPA3; RP-A p14
• NCBI Protein Information: replication protein A 14 kDa subunit
• UniProt Protein Name: Replication protein A 14 kDa subunit
• Protein Family: Replication protein
• UniProt Gene Name: RPA3
• UniProt Synonym Gene Names: REPA3; RPA14; RP-A p14; RF-A protein 3
• UniProt Entry Name: RFA3_HUMAN

Uniprot Description

RPA3: Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: nucleoplasm; DNA replication factor A complex

Molecular Function: protein binding; damaged DNA binding; single-stranded DNA binding

Biological Process: mismatch repair; regulation of mitotic cell cycle; DNA strand elongation during DNA replication; DNA repair; double-strand break repair via homologous recombination; regulation of cell proliferation; telomere maintenance via semi-conservative replication; DNA recombinase assembly; nucleotide-excision repair; base-excision repair; transcription-coupled nucleotide-excision repair; double-strand break repair; telomere maintenance via recombination; nucleotide-excision repair, DNA damage removal; nucleotide-excision repair, DNA gap filling; mitotic cell cycle; DNA replication; telomere maintenance; G1/S transition of mitotic cell cycle