PLEKHO1 cdna clone

PLEKHO1

Cat. Num. AAA200957

• Synonyms: PLEKHO1; CKIP-1; CKIP1; JBP; OC120; PLEKHO1 cdna clone

• Form/Format: Lyophilized
• Sequence: Nucleotide Sequence: ATGATGAAGAAGAACAATTCCGCCAAGCGGGGACCTCAGGATGGAAACCAGCAGCCTGCACCGCCCGAGAAGGTCGGCTGGGTCCGGAAATTCTGCGGGAAAGGGATTTTCAGGGAGATTTGGAAAAACCGCTATGTGGTGCTGAAAGGGGACCAGCTCTACATCTCTGAGAAGGAGGTAAAAGATGAGAAAAATATTCAAGAGGTATTTGACCTGAGTGACTATGAGAAGTGTGAAGAGCTCCGGAAGTCCAAGAGCAGGAGCAAGAAAAATCATAGCAAGTTTACTCTTGCCCACTCCAAACAGCCCGGTAACACGGCACCCAACCTGATCTTCCTGGCAGTGAGTCCAGAAGAGAAGGAATCGTGGATCAATGCCCTCAACTCTGCCATCACCCGAGCCAAGAACCGTATCTTGGATGAGGTCACCGTTGAGGAGGACAGCTATCTTGCCCATCCCACTCGAGACAGGGCAAAAATCCAGCACTCCCGCCGCCCCCCAACAAGGGGACACCTAATGGCTGTGGCTTCCACCTCTACCTCGGATGGGATGCTGACCTTGGACTTGATCCAAGAGGAAGACCCTTCCCCTGAGGAACCAACCTCTTGTGCTGAGAGCTTTCGGGTTGACCTGGACAAGTCTGTGGCCCAGCTGGCAGGGAGCCGGCGGAGAGCGGACTCAGACCGCATCCAGCCCTCCGCAGACCGGGCAAGCAGTCTCTCCCGACCTTGGGAAAAAACAGACAAAGGGGCCACCTACACCCCCCAGGCACCCAAGAAGTTGACGCCCACAGAGAAAGGCCGCTGCGCCTCCCTGGAGGAGATCCTATCTCAGCGGGATGCTGCCTCTGCCCGCACCCTCCAGCTGCGGGCTGAGGAACCCCCAACCCCTGCCCTCCCCAACCCGGGGCAGCTGTCCCGGATCCAGGACCTGGTAGCAAGGAAACTGGAGGAGACTCAGGAGCTTCTGGCAGAGGTTCAGGGACTGGGAGATGGGAAGCGAAAGGCCAAGGACCCCCCTCGGTCTCCGCCGGATTCTGAGTCAGAGCAGCTGCTGCTGGAGACGGAACGGCTGCTGGGAGAGGCATCATCGAATTGGAGCCAGGCAAAGAGGGTGCTGCAGGAGGTCAGGGAGCTGAGAGACCTGTACAGACAGATGGACCTGCAGACCCCGGACTCCCACCTCAGACAGACCACCCCGCACAGTCAGTACCGGAAGAGCCTGATGTGA; ; Translation Sequence: MMKKNNSAKR GPQDGNQQPA PPEKVGWVRK FCGKGIFREI WKNRYVVLKG DQLYISEKEVKDEKNIQEVF DLSDYEKCEE LRKSKSRSKK NHSKFTLAHS KQPGNTAPNL IFLAVSPEEKESWINALNSA ITRAKNRILD EVTVEEDSYL AHPTRDRAKI QHSRRPPTRG HLMAVASTSTSDGMLTLDLI QEEDPSPEEP TSCAESFRVD LDKSVAQLAG SRRRADSDRI QPSADRASSLSRPWEKTDKG ATYTPQAPKK LTPTEKGRCA SLEEILSQRD AASARTLQLR AEEPPTPALPNPGQLSRIQD LVARKLEETQ ELLAEVQGLG DGKRKAKDPP RSPPDSESEQ LLLETERLLGEASSNWSQAK RVLQEVRELR DLYRQMDLQT PDSHLRQTTP HSQYRKSLM
• Sequence Length: 409

• Species: Human
• Chromosome Location: 1q21.2
• OMIM Reference Number: 608335
• cDNA Size: 1230bp
• Vector Description: This shuttle vector contains the complete ORF. It is inseted Nde I to Hind III. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.
• Vector: (puc19-derived cloning vector)
• Preparation Before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid.
• Preparation and Storage: Store the plasmid at -20 degree C.

Related Product Information for PLEKHO1 cdna clone

PLEKHO1 plays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein (CP). It may function to target CK2 to the plasma membrane thereby serving as an adapter to facilitate the phosphorylation of CP by protein kinase 2 (CK2).

Product Categories/Family for PLEKHO1 cdna clone

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 20149626
• NCBI Accession #: NP_057358.2
• NCBI GenBank Nucleotide #: NM_016274.5
• UniProt Accession #: Q53GL0
• NCBI Official Full Name: pleckstrin homology domain-containing family O member 1 isoform a
• UniProt Protein Name: Pleckstrin homology domain-containing family O member 1
• Protein Family: Pleckstrin homology domain-containing family
• UniProt Gene Name: PLEKHO1
• UniProt Synonym Gene Names: CKIP1; OC120; PH domain-containing family O member 1; JBP; CK2-interacting protein 1; CKIP-1
• UniProt Entry Name: PKHO1_HUMAN

Uniprot Description

PLEKHO1: Plays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein (CP). May function to target CK2 to the plasma membrane thereby serving as an adapter to facilitate the phosphorylation of CP by protein kinase 2 (CK2). Appears to target ATM to the plasma membrane. Appears to also inhibit tumor cell growth by inhibiting AKT- mediated cell-survival. Also implicated in PI3K-regulated muscle differentiation, the regulation of AP-1 activity (plasma membrane bound AP-1 regulator that translocates to the nucleus) and the promotion of apoptosis induced by tumor necrosis factor TNF. When bound to PKB, it inhibits it probably by decreasing PKB level of phosphorylation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1q21.2

Cellular Component: cytoplasm; plasma membrane; nucleus

Molecular Function: protein binding