BCAS2 cdna clone

BCAS2

Cat. Num. AAA200577

• Gene Names: BCAS2; DAM1; SPF27; Snt309
• Synonyms: BCAS2; DAM1; Snt309; SPF27; BCAS2 cdna clone

• Form/Format: Lyophilized
• Sequence: Nucleotide Sequence: ATGGCGGGCA CAGGTTTGGT GGCTGGAGAG GTTGTGGTGG ATGCGCTGCC GTATTTTGAT CAAGGTTATG AAGCCCCTGG TGTGCGGGAA GCGGCTGCAG CGCTGGTGGA GGAGGAAACT CGCAGATACC GACCTACTAA GAACTACCTG AGCTACCTGA CAGCCCCGGA TTATTCTGCC TTTGAAACTG ACATAATGAG AAATGAATTT GAAAGACTGG CTGCTCGACA ACCAATTGAA TTGCTCAGTA TGAAACGATA TGAGCTTCCA GCCCCCTCCT CTGGTCAAAA AAATGACATT ACTGCATGGC AAGAATGTGT AAACAATTCT ATGGCCCAGT TAGAGCATCA AGCAGTTAGA ATTGAGAATC TGGAACTAAT GTCACAGCAT GGATGTAATG CCTGGAAAGT ATACAATGAA AATCTAGTTC ATATGATTGA ACACGCACAG AAGGAACTTC AGAAGTTAAG AAAACATATT CAAGATTTAA ACTGGCAGAG AAAGAACATG CAACTCACAG CTGGATCTAA ATTGAGAGAA ATGGAGTCAA ATTGGGTATC CCTGGTCAGT AAGAATTATG AGATTGAACG GACTATTGTT CAGCTAGAAA ATGAAATCTA TCAAATTAAG CAGCAACATG GAGAGGCAAA CAAAGAAAAC ATCCGGCAAG ACTTCTGA; ; Translation Sequence: MAGTGLVAGE VVVDALPYFD QGYEAPGVRE AAAALVEEET RRYRPTKNYL SYLTAPDYSA FETDIMRNEF ERLAARQPIE LLSMKRYELP APSSGQKNDI TAWQECVNNS MAQLEHQAVR IENLELMSQH GCNAWKVYNE NLVHMIEHAQ KELQKLRKHI QDLNWQRKNM QLTAGSKLRE MESNWVSLVS KNYEIERTIV QLENEIYQIK QQHGEANKEN IRQDF
• Sequence Length: 225

• Species: Human
• Chromosome Location: 1q13.2
• OMIM Reference Number: 605783
• cDNA Size: 678bp
• Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.
• Vector: (puc19-derived cloning vector)
• Preparation Before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid.
• Preparation and Storage: Store the plasmid at -20 degree C.

Related Product Information for BCAS2 cdna clone

BCAS2, also known as pre-mRNA-splicing factor SPF27, is a ubiquitously expressed nuclear protein that was originally identified as being overexpressed in various breast cancer cell lines. It is now known to be a component of the spliceosome, participating in the removal of introns from mRNA precursors. BCAS2 specifically interacts (in a ligand-independent manner) with TRbeta (thyroid hormone receptor beta), ERalpha (estrogen receptor alpha), ERbeta, PR (progesterone receptor) and PPARgamma (peroxisome proliferator-activated receptor gamma). This protein functions as an ER co-activator and is capable of enhancing ER-mediated transcription. This suggests that BCAS2 is involved in the development of breast cancer.

Product Categories/Family for BCAS2 cdna clone

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 5031653
• NCBI GeneID: 10286
• NCBI Accession #: NP_005863.1
• NCBI GenBank Nucleotide #: NM_005872.2
• UniProt Accession #: O75934
• NCBI Official Full Name: pre-mRNA-splicing factor SPF27
• NCBI Official Synonym Full Names: BCAS2 pre-mRNA processing factor
• NCBI Official Symbol: BCAS2
• NCBI Official Synonym Symbols: DAM1; SPF27; Snt309
• NCBI Protein Information: pre-mRNA-splicing factor SPF27
• UniProt Protein Name: Pre-mRNA-splicing factor SPF27
• UniProt Gene Name: BCAS2
• UniProt Synonym Gene Names: DAM1
• UniProt Entry Name: SPF27_HUMAN

Uniprot Description

BCAS2: Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. Belongs to the SPF27 family.

Protein type: RNA splicing; Spliceosome; Nuclear receptor co-regulator; Nucleolus

Chromosomal Location of Human Ortholog: 1p13.2

Cellular Component: nucleoplasm; spliceosome; DNA replication factor A complex; nucleolus; nucleus; cell junction

Molecular Function: protein binding

Biological Process: RNA splicing, via transesterification reactions; nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression