SDS-Page
BCAS2 recombinant protein
BCAS2, 1-225aa, Human, His tag, E Coli
Gene Names
BCAS2; DAM1; SPF27; Snt309
Applications
SDS-Page
Purity
> 85% by SDS-PAGE
Synonyms
BCAS2; 1-225aa; Human; His tag; E Coli; pre-mRNA-splicing factor SPF27; DAM1; Snt307; SPF27; BCAS2 recombinant protein
Host
E Coli
Purity/Purification
> 85% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 5mM DTT, 0.2M NaCl.
Concentration
0.25 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMAGT GLVAGEVVVD ALPYFDQGYE APGVREAAAA LVEEETRRYR PTKNYLSYLT APDYSAFETD IMRNEFERLA ARQPIELLSM KRYELPAPSS GQKNDITAWQ ECVNNSMAQL EHQAVRIENL ELMSQHGCNA WKVYNENLVH MIEHAQKELQ KLRKHIQDLN WQRKNMQLTA GSKLREMESN WVSLVSKNYE IERTIVQLEN EIYQIKQQHG EANKENIRQD F
Sequence Length
225
Applicable Applications for BCAS2 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for BCAS2 recombinant protein
BCAS2, also known as pre-mRNA-splicing factor SPF27, is a ubiquitously expressed nuclear protein that was originally identified as being overexpressed in various breast cancer cell lines. It is now known to be a component of the spliceosome, participating in the removal of introns from mRNA precursors. BCAS2 specifically interacts (in a ligand-independent manner) with TRbeta (thyroid hormone receptor beta), ERalpha (estrogen receptor alpha), ERbeta, PR (progesterone receptor) and PPARgamma (peroxisome proliferator-activated receptor gamma). This protein functions as an ER co-activator and is capable of enhancing ER-mediated transcription. This suggests that BCAS2 is involved in the development of breast cancer. Recombinant human BCAS2 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for BCAS2 recombinant protein
References
Qi C., et al. (2005) Biochem Biophys Res Commun. 328 (2):393-8. ; Kuo PC., et al. (2009) Cancer Res. 69 (23):8877-85.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30.3kDa (261aa), confirmed by MALDI-TOF
NCBI Official Full Name
pre-mRNA-splicing factor SPF27
NCBI Official Synonym Full Names
BCAS2, pre-mRNA processing factor
NCBI Official Symbol
BCAS2
NCBI Official Synonym Symbols
DAM1; SPF27; Snt309
NCBI Protein Information
pre-mRNA-splicing factor SPF27
UniProt Protein Name
Pre-mRNA-splicing factor SPF27
UniProt Gene Name
BCAS2
UniProt Synonym Gene Names
DAM1
Uniprot Description
Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR).
Research Articles on BCAS2
Similar Products
Product Notes
The BCAS2 bcas2 (Catalog #AAA203900) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's BCAS2 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the BCAS2 bcas2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMAGT GLVAGEVVVD ALPYFDQGYE APGVREAAAA LVEEETRRYR PTKNYLSYLT APDYSAFETD IMRNEFERLA ARQPIELLSM KRYELPAPSS GQKNDITAWQ ECVNNSMAQL EHQAVRIENL ELMSQHGCNA WKVYNENLVH MIEHAQKELQ KLRKHIQDLN WQRKNMQLTA GSKLREMESN WVSLVSKNYE IERTIVQLEN EIYQIKQQHG EANKENIRQD F. It is sometimes possible for the material contained within the vial of "BCAS2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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