CDK7 & CCNH & MNAT1 Heterotrimer Recombinant Protein | CDK7 recombinant protein

Recombinant Human CDK7 & CCNH & MNAT1 Heterotrimer Protein

Cat. Num. AAA2545011

• Gene Names: CDK7; CAK; CAK1; HCAK; MO15; STK1; CDKN7; p39MO15
• Purity: > 85 % as determined by SDS-PAGE
• Synonyms: CDK7 & CCNH & MNAT1 Heterotrimer; Recombinant Human CDK7 & CCNH & MNAT1 Heterotrimer Protein; CDK7 (cyclin-dependent kinase 7); CCNH (cyclin H) & MNAT1 (menage a trois homolog 1; cyclin H assembly factor (Xenopus laevis)) Heterotrimer; CDK7 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 85 % as determined by SDS-PAGE
• Form/Format: Supplied as sterile PBS, pH 7.4
• Sequence Length: 305

• Application Notes: The recombinant heterotrimer of human CDK7/CCNH/MNAT1 comprises 1032 (364 + 341 + 327) amino acids and has a calculated molecular mass of 118.8 (41.2 + 39.7 + 37.9) kDa. The apparent molecular mass of rh CDK7/CCNH/MNAT1 heterotrimer is approximately 25, 38 & 44 kDa respectively in SDS-PAGE under reducing conditions.
• Predicted N Terminal: His & His & His
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for CDK7 recombinant protein

A DNA sequence encoding the human CDK7 (P50613) (Ala 2-Phe 346) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 1; A DNA sequence encoding the human CCNH (P51946) (Tyr 2-Leu 323) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 2. A DNA sequence encoding the human MNAT1 (P51948) (Asp 2-Ser 309) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.

NCBI and Uniprot Product Information

• NCBI GI #: 1022435305
• NCBI GeneID: 1022
• NCBI Accession #: NP_001310998.1
• NCBI GenBank Nucleotide #: NM_001324069.1
• UniProt Accession #: P50613; Q9BS60; Q9UE19
• Molecular Weight: 39,038 Da
• NCBI Official Full Name: cyclin-dependent kinase 7 isoform 2
• NCBI Official Synonym Full Names: cyclin dependent kinase 7
• NCBI Official Symbol: CDK7
• NCBI Official Synonym Symbols: CAK; CAK1; HCAK; MO15; STK1; CDKN7; p39MO15
• NCBI Protein Information: cyclin-dependent kinase 7
• UniProt Protein Name: Cyclin-dependent kinase 7
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: CDK7
• UniProt Synonym Gene Names: CAK; CAK1; CDKN7; MO15; STK1; p39 Mo15

NCBI Description

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This protein forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle. [provided by RefSeq, Jul 2008]

Uniprot Description

CDK7: a protein kinase of the CDK family. Forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). Activates the cyclin-associated kinases CDK1, -2, -4 and -6. An essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. Serves as a direct link between the regulation of transcription and the cell cycle. Phosphorylates and activates RNA polymerase II, allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.

Protein type: CDK family; CDK/CDK7 subfamily; CDK7 subfamily; CMGC group; Cell cycle regulation; EC 2.7.11.22; EC 2.7.11.23; Kinase, protein; Nuclear receptor co-regulator; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 5q13.2

Cellular Component: cytoplasm; holo TFIIH complex; mitochondrion; nucleoplasm; nucleus

Molecular Function: DNA-dependent ATPase activity; kinase activity; protein binding; protein C-terminus binding; protein kinase activity; protein serine/threonine kinase activity; RNA polymerase subunit kinase activity

Biological Process: cell cycle arrest; cell proliferation; G1/S transition of mitotic cell cycle; G2/M transition of mitotic cell cycle; mRNA capping; nucleotide-excision repair, preincision complex assembly; positive regulation of transcription from RNA polymerase II promoter; regulation of cyclin-dependent protein kinase activity; RNA elongation from RNA polymerase I promoter; RNA elongation from RNA polymerase II promoter; snRNA transcription from RNA polymerase II promoter; termination of RNA polymerase I transcription; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase I promoter; transcription initiation from RNA polymerase II promoter; transcription-coupled nucleotide-excision repair