AMPK (G1/B2/A1) Heterotrimer Recombinant Protein | AMPK recombinant protein

Recombinant Human AMPK (G1/B2/A1) Heterotrimer Protein

Cat. Num. AAA2545014

• Gene Names: PRKAG1; AMPKG
• Purity: > 94 % as determined by SDS-PAGE
• Synonyms: AMPK (G1/B2/A1) Heterotrimer; Recombinant Human AMPK (G1/B2/A1) Heterotrimer Protein; AMPK; 5' AMP-activated protein kinase; 5' adenosine monophosphate-activated protein kinase. Heterotrimer of alpha 1 catalytic subunit; beta 2 non-catalytic subunit and gamma 1 non-catalytic subunit.; AMPK recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 94 % as determined by SDS-PAGE
• Form/Format: Supplied as sterile 50mM Tris, 200mM NaCl, 2mM GSH, 1mM EDTA, 1mM DTT, 10% gly, pH 7.4
• Sequence Length: 340

• Application Notes: The recombinant heterotrimer of human AMPK (PRKAG1 / PRKAB2 / PRKAA1) has a calculated molecular mass of 160 (38+30+92) KDa. The apparent molecular mass is approximately 35, 37 & 95 KDa respectively in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met & Met & His
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for AMPK recombinant protein

A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB2 (O43741) (Met 1-Ile 272), constructed the plasmid 2; A DNA sequence encoding the human PRKAA1 (Q13131-1) (Met 1-Gln 559) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.

NCBI and Uniprot Product Information

• NCBI GI #: 332000015
• NCBI GeneID: 5571
• NCBI Accession #: NP_001193638.1
• NCBI GenBank Nucleotide #: NM_001206709.1
• UniProt Accession #: P54619; Q8N7V9; B4DDT7
• Molecular Weight: 38,533 Da
• NCBI Official Full Name: 5'-AMP-activated protein kinase subunit gamma-1 isoform 3
• NCBI Official Synonym Full Names: protein kinase AMP-activated non-catalytic subunit gamma 1
• NCBI Official Symbol: PRKAG1
• NCBI Official Synonym Symbols: AMPKG
• NCBI Protein Information: 5'-AMP-activated protein kinase subunit gamma-1
• UniProt Protein Name: 5'-AMP-activated protein kinase subunit gamma-1
• UniProt Gene Name: PRKAG1
• UniProt Synonym Gene Names: AMPK gamma1; AMPK subunit gamma-1; AMPKg

NCBI Description

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

AMPKG1: a non-catalytic subunit of AMPK, a highly conserved kinase of the CAMKL family. The holoenzyme consists of a catalytic subunit (alpha) and two regulatory subunits (beta, gamma). Each subunit exists as multiple genetic isoforms giving rise to 12 different possible combinations of holoenzyme. Plays a key role in regulation of energy homeostasis. Activated by an elevated AMP-ATP ratio due to environmental stress, such as heat shock, hypoxia and ischemia. AMPK phosphorylates and regulates many metabolic enzymes.

Protein type: Autophagy; Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 12q13.12

Cellular Component: AMP-activated protein kinase complex; cytosol; membrane; nucleoplasm

Molecular Function: ADP binding; AMP binding; AMP-activated protein kinase activity; ATP binding; cAMP-dependent protein kinase activity; cAMP-dependent protein kinase regulator activity; protein binding; protein kinase activity; protein kinase binding

Biological Process: cell cycle arrest; macroautophagy; positive regulation of protein kinase activity; protein amino acid phosphorylation; regulation of glycolysis; signal transduction; spermatogenesis