Rat Cyclin-dependent kinase 7 ELISA Kit | Cdk7 elisa kit

Rat Cyclin-dependent kinase 7 ELISA Kit

Cat. Num. AAA288335

• Reactivity: Rat
• Synonyms: Cyclin-dependent kinase 7; Rat Cyclin-dependent kinase 7 ELISA Kit; Cdk7 elisa kit

• Reactivity: Rat
• Specificity: This assay recognizes recombinant and natural rat Cyclin-dependent kinase 7. No significant cross-reactivity or interference was observed.
• Sequence Length: 346

• Assay Type: Sandwich
• Detection Range: 0.312-20 ng/ml
• Sensitivity: 0.171 ng/mL
• Intra-Assay CV: <=4.7%
• Inter-Assay CV: <=8.3%
• Recovery: 98%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Cdk7 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin-dependent kinase 7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Cyclin-dependent kinase 7 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cyclin-dependent kinase 7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Cyclin-dependent kinase 7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 68566250
• NCBI GeneID: 171150
• UniProt Accession #: P51952
• Molecular Weight: 37,141 Da
• NCBI Official Full Name: Cyclin-dependent kinase 7
• NCBI Official Synonym Full Names: cyclin-dependent kinase 7
• NCBI Official Symbol: Cdk7
• NCBI Protein Information: cyclin-dependent kinase 7
• UniProt Protein Name: Cyclin-dependent kinase 7
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: Cdk7
• UniProt Synonym Gene Names: Cak; Cak1; Mo15; P39 Mo15
• UniProt Entry Name: CDK7_RAT

NCBI Description

human homolog is a subunit of trimeric cdk-activating kinase CAK, which inhibits RNA polymerase II transcription by reducing RNAPII and TFIIF entry into the preinitiation complex [RGD, Feb 2006]

Uniprot Description

CDK7: a protein kinase of the CDK family. Forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). Activates the cyclin-associated kinases CDK1, -2, -4 and -6. An essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. Serves as a direct link between the regulation of transcription and the cell cycle. Phosphorylates and activates RNA polymerase II, allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.

Protein type: EC 2.7.11.22; EC 2.7.11.23; Kinase, protein; Cell cycle regulation; Nuclear receptor co-regulator; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; CMGC group; CDK family; CDK7 subfamily; CDK/CDK7 subfamily

Cellular Component: cyclin-dependent protein kinase holoenzyme complex; cytoplasm; cytoskeleton; cytosol; holo TFIIH complex; membrane; mitochondrion; nucleus; perinuclear region of cytoplasm

Molecular Function: ATP binding; cyclin-dependent protein kinase activity; DNA-dependent ATPase activity; kinase activity; protein C-terminus binding; protein complex binding; protein kinase activity; RNA polymerase subunit kinase activity

Biological Process: cell division; DNA repair; meiotic cell cycle; positive regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; regulation of cell cycle; regulation of transcription, DNA-dependent; transcription from RNA polymerase II promoter