Canine Cyclin Dependent Kinase 7 ELISA Kit | CDK-7 elisa kit

Canine Cyclin Dependent Kinase 7 ELISA Kit

Cat. Num. AAA734137

• Gene Names: CDK7; CAK1; HCAK; MO15; STK1; CDKN7; p39MO15
• Reactivity: Canine
• Synonyms: Cyclin Dependent Kinase 7; Canine Cyclin Dependent Kinase 7 ELISA Kit; CDK-7 elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 5.0-100 U/L
• Sensitivity: 1.0 U/L
• Intended Uses: This CDK-7 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine CDK-7. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CDK-7 elisa kit

Principle of the Assay: CDK-7 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CDK-7 antibody and an CDK-7-HRP conjugate. The assay sample and buffer are incubated together with CDK-7-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CDK-7 concentration since CDK-7 from samples and CDK-7-HRP conjugate compete for the anti-CDK-7 antibody binding site. Since the number of sites is limited, as more sites are occupied by CDK-7 from the sample, fewer sites are left to bind CDK-7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDK-7 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CDK-7 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 4502743
• NCBI GeneID: 1022
• NCBI Accession #: NP_001790.1
• NCBI GenBank Nucleotide #: NM_001799.3
• Molecular Weight: 39,038 Da
• NCBI Official Full Name: cyclin-dependent kinase 7
• NCBI Official Synonym Full Names: cyclin-dependent kinase 7
• NCBI Official Symbol: CDK7
• NCBI Official Synonym Symbols: CAK1; HCAK; MO15; STK1; CDKN7; p39MO15
• NCBI Protein Information: cyclin-dependent kinase 7; CAK; p39 Mo15; protein kinase; 39 KDa protein kinase; kinase subunit of CAK; CDK-activating kinase 1; serine/threonine kinase stk1; cell division protein kinase 7; serine/threonine protein kinase 1; serine/threonine-protein kinase 1; serine/threonine protein kinase MO15; homolog of Xenopus MO15 Cdk-activating kinase; TFIIH basal transcription factor complex kinase subunit; cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase)
• UniProt Protein Name: Cyclin-dependent kinase 7
• UniProt Gene Name: CDK7
• UniProt Synonym Gene Names: CAK; CAK1; CDKN7; MO15; STK1; p39 Mo15
• UniProt Entry Name: CDK7_HUMAN

NCBI Description

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This protein forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle. [provided by RefSeq, Jul 2008]

Uniprot Description

CDK7: a protein kinase of the CDK family. Forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). Activates the cyclin-associated kinases CDK1, -2, -4 and -6. An essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. Serves as a direct link between the regulation of transcription and the cell cycle. Phosphorylates and activates RNA polymerase II, allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.

Protein type: Protein kinase, CMGC; Cell cycle regulation; EC 2.7.11.23; Nuclear receptor co-regulator; Kinase, protein; EC 2.7.11.22; Protein kinase, Ser/Thr (non-receptor); CMGC group; CDK family; CDK7 subfamily; CDK/CDK7 subfamily

Chromosomal Location of Human Ortholog: 5q12.1

Cellular Component: nucleoplasm; holo TFIIH complex; mitochondrion; perinuclear region of cytoplasm; cytoplasm; nucleus

Molecular Function: RNA polymerase subunit kinase activity; protein C-terminus binding; DNA-dependent ATPase activity; protein binding; androgen receptor binding; cyclin-dependent protein kinase activity; transcription coactivator activity; ATP binding; protein kinase activity

Biological Process: transcription from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; positive regulation of transcription, DNA-dependent; regulation of gene expression, epigenetic; protein amino acid phosphorylation; mRNA capping; negative regulation of gene expression, epigenetic; transcription-coupled nucleotide-excision repair; nucleotide-excision repair, DNA damage removal; cell cycle arrest; G2/M transition of mitotic cell cycle; transcription initiation from RNA polymerase II promoter; RNA elongation from RNA polymerase I promoter; transcription from RNA polymerase I promoter; DNA repair; termination of RNA polymerase I transcription; cell proliferation; nucleotide-excision repair; cell division; androgen receptor signaling pathway; RNA elongation from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; gene expression; mitotic cell cycle; regulation of cyclin-dependent protein kinase activity; transcription initiation from RNA polymerase I promoter; G1/S transition of mitotic cell cycle