Human Neural Cadherin ELISA Kit | CDH2 elisa kit

Human Neural Cadherin ELISA Kit

Cat. Num. AAA762775

• Reactivity: Human
• Synonyms: Neural Cadherin; Human Neural Cadherin ELISA Kit; N-Cadherin/NCAD/CDH2/Cadherin-2/CD325/CDHN/CDw325/Cadherin 2 Type 1; CDH2 elisa kit

• Reactivity: Human
• Sequence Length: 2662

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for CDH2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human N-Cadherin antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human N-Cadherin antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human N-Cadherin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human N-Cadherin can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 674567416
• Molecular Weight: 16,831 Da
• NCBI Official Full Name: neural cadherin
• UniProt Protein Name: Protein MGF 100-2L
• Protein Family: Cadherin
• UniProt Gene Name: Ken-162
• UniProt Entry Name: 1002L_ASFK5

Uniprot Description

ERK1: a serine/threonine kinase of the GMGC group that plays a critical role in the regulation of cell growth and differentiation. ERK1 (MAPK3) and ERK2 (MAPK1) play central roles in MAPK cascades and are activated by a wide variety of extracellular signals including growth and neurotrophic factors, cytokines, hormones and neurotransmitters. Depending on the cellular context, MAPK cascades mediate diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. MAPK cascades also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine residues at the sequence T*EY* by upstream MAP kinase kinases, MEK1 and -2. Phosphorylation of both the threonine and tyrosine are required for activity. This phosphorylation causes dramatic conformational changes, which enable full activation and interaction of MAPK1/ERK2 with its substrates.

Protein type: CMGC group; EC 2.7.11.24; ERK subfamily; Kinase, protein; MAPK family; MAPK/ERK subfamily; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: caveola; cytoskeleton; cytosol; early endosome; focal adhesion; Golgi apparatus; late endosome; microtubule cytoskeleton; mitochondrion; nuclear envelope; nucleoplasm; nucleus

Molecular Function: kinase activity; MAP kinase activity; phosphatase binding; protein binding; protein serine/threonine kinase activity

Biological Process: activation of MAPK activity; axon guidance; BMP signaling pathway; DNA damage induced protein phosphorylation; fibroblast growth factor receptor signaling pathway; MAPKKK cascade; phosphorylation; platelet activation; positive regulation of cyclase activity; positive regulation of histone acetylation; positive regulation of histone phosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of telomerase activity; positive regulation of telomere maintenance via telomerase; positive regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; regulation of cytoskeleton organization and biogenesis; regulation of phosphoinositide 3-kinase cascade; regulation of stress-activated MAPK cascade; regulation of transcription factor activity; transcription initiation from RNA polymerase I promoter