Mouse Cadherin-3 ELISA Kit | Cdh3 elisa kit

Mouse Cadherin-3 ELISA Kit

Cat. Num. AAA763670

• Gene Names: CDH3; CDHP; HJMD; PCAD
• Reactivity: Mouse
• Synonyms: Cadherin-3; Mouse Cadherin-3 ELISA Kit; Cdh3/P-Cadherin/CAD3/Cadherin-3/CDH3/CDHP/HJMD/PCAD/cadherin 3; P-cadherin (placental)/cadherin 3; type 1; P-cadherin (placental)/cadherin-3/CDHPcalcium-dependent adhesion protein; placental/HJMD/PCADP-cadherin/Placental cadherin; Cdh3 elisa kit

• Reactivity: Mouse
• Sequence Length: 829

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Cdh3 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Cdh3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Cdh3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Cdh3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Cdh3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 14589891
• NCBI GeneID: 1001
• NCBI Accession #: NP_001784.2
• NCBI GenBank Nucleotide #: NM_001793.5
• Molecular Weight: 86,552 Da
• NCBI Official Full Name: cadherin-3 isoform 1 preproprotein
• NCBI Official Synonym Full Names: cadherin 3
• NCBI Official Symbol: CDH3
• NCBI Official Synonym Symbols: CDHP; HJMD; PCAD
• NCBI Protein Information: cadherin-3
• UniProt Protein Name: Cadherin-3
• Protein Family: Cadherin
• UniProt Gene Name: CDH3
• UniProt Synonym Gene Names: CDHP; P-cadherin
• UniProt Entry Name: CADH3_HUMAN

NCBI Description

This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. This gene is located in a gene cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene are associated with hypotrichosis with juvenile macular dystrophy and ectodermal dysplasia, ectrodactyly, and macular dystrophy syndrome (EEMS). [provided by RefSeq, Nov 2015]

Uniprot Description

CDH3: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Interacts with CDCP1. Expressed in some normal epithelial tissues and in some carcinoma cell lines. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: cell-cell adherens junction; cytoplasm; integral to membrane; plasma membrane

Molecular Function: calcium ion binding

Biological Process: cell adhesion; cell-cell adhesion; hair cycle process; homophilic cell adhesion; intercellular junction assembly and maintenance; keratinization; negative regulation of catagen; negative regulation of transforming growth factor-beta2 production; positive regulation of insulin-like growth factor receptor signaling pathway; positive regulation of melanin biosynthetic process; positive regulation of monophenol monooxygenase activity; response to drug; retinal homeostasis; visual perception; Wnt receptor signaling pathway through beta-catenin; wound healing

Disease: Ectodermal Dysplasia, Ectrodactyly, And Macular Dystrophy Syndrome; Hypotrichosis, Congenital, With Juvenile Macular Dystrophy