Human Type-1 angiotensin II receptor ELISA Kit | AGTR1 elisa kit

Human Type-1 angiotensin II receptor ELISA Kit

Cat. Num. AAA762100

• Gene Names: AGTR1; AT1; AG2S; AT1B; AT1R; AT1AR; AT1BR; AT2R1; HAT1R; AGTR1B
• Reactivity: Human
• Synonyms: Type-1 angiotensin II receptor; Human Type-1 angiotensin II receptor ELISA Kit; ANG II R1/AGTR1 (Angiotensin II Receptor 1)/AT1R/AG2S/AG2-S/AGTR1A/AGTR1B/AT1/AT1B/AT2R1/AT2-R1/AT2R1A/AT2R1B/HAT1R/Angiotensin II receptor; type; AGTR1 elisa kit

• Reactivity: Human
• Sequence Length: 359

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for AGTR1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human ANGIIR1antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human ANGIIR1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ANGIIR1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman ANGIIR1can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 999394
• NCBI GeneID: 185
• Molecular Weight: 41,061 Da
• NCBI Official Full Name: type 1 angiotensin II receptor
• NCBI Official Synonym Full Names: angiotensin II receptor type 1
• NCBI Official Symbol: AGTR1
• NCBI Official Synonym Symbols: AT1; AG2S; AT1B; AT1R; AT1AR; AT1BR; AT2R1; HAT1R; AGTR1B
• NCBI Protein Information: type-1 angiotensin II receptor
• UniProt Protein Name: Type-1 angiotensin II receptor
• Protein Family: Type-1 angiotensin II receptor
• UniProt Gene Name: AGTR1
• UniProt Synonym Gene Names: AGTR1A; AGTR1B; AT2R1; AT2R1B; AT1
• UniProt Entry Name: AGTR1_HUMAN

NCBI Description

Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors. This gene encodes the type 1 receptor which is thought to mediate the major cardiovascular effects of angiotensin II. This gene may play a role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. It was previously thought that a related gene, denoted as AGTR1B, existed; however, it is now believed that there is only one type 1 receptor gene in humans. Multiple alternatively spliced transcript variants have been reported for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

AT1: receptor for angiotensin II. Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. Mediates the major cardiovascular effects of angiotensin II. May play role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Protein type: Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral; Receptor, GPCR

Chromosomal Location of Human Ortholog: 3q24

Cellular Component: cytosol; integral to membrane; integral to plasma membrane; intracellular; plasma membrane

Molecular Function: angiotensin type I receptor activity; angiotensin type II receptor activity; bradykinin receptor binding; protein binding; protein heterodimerization activity

Biological Process: calcium-mediated signaling; elevation of cytosolic calcium ion concentration; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); G-protein coupled receptor protein signaling pathway; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); kidney development; positive regulation of cellular protein metabolic process; positive regulation of inflammatory response; positive regulation of NAD(P)H oxidase activity; positive regulation of phospholipase A2 activity; regulation of cell growth; regulation of cell proliferation; regulation of inflammatory response; regulation of systemic arterial blood pressure by renin-angiotensin; regulation of vasoconstriction; regulation of vasodilation; renin-angiotensin regulation of aldosterone production; renin-angiotensin regulation of blood vessel size; Rho protein signal transduction

Disease: Hypertension, Essential; Renal Tubular Dysgenesis