Typical Testing Data/Standard Curve (for reference only)
(Mouse CD200 PicoKine ELISA Kit standard curve )
Mouse CD200 ELISA Kit | CD200 elisa kit
Mouse CD200 PicoKine ELISA Kit
Gene Names
Cd200; OX2; Mox2
Reactivity
Mouse
Synonyms
CD200; Mouse CD200 PicoKine ELISA Kit; OX-2 membrane glycoprotein; CD200 molecule; antigen identified by monoclonal antibody MRC OX-2; CD_antigen; CD200 antigen; MOX1; MOX2; MRC OX-2 antigen; My033; CD200 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse CD200
Sequence Length
278
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
31.2pg/ml-2000pg/ml
Sensitivity
< 10 pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Mouse CD200 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Mouse CD200 PicoKine ELISA Kit standard curve )
Related Product Information for CD200 elisa kit
Principle of the assay: mouse CD200 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD200 has been precoated onto 96-well plates. Standards(NSO, Q31-G232) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD200 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD200 amount of sample captured in plate.
Background: OX-2 membrane glycoprotein, also known as CD200 is a human protein encoded by the CD200 gene. This gene is mapped to 3q13.2. The protein encoded by this gene is a type-1 membrane glycoprotein, which contains two immunoglobulin domains, and thus belongs to the immunoglobulin superfamily. Studies of the related genes in mouse and rat suggest that this gene may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. It is suggested that CD200-CD200R signaling plays a central role in macrophage fusion and osteoclast formation.
Background: OX-2 membrane glycoprotein, also known as CD200 is a human protein encoded by the CD200 gene. This gene is mapped to 3q13.2. The protein encoded by this gene is a type-1 membrane glycoprotein, which contains two immunoglobulin domains, and thus belongs to the immunoglobulin superfamily. Studies of the related genes in mouse and rat suggest that this gene may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. It is suggested that CD200-CD200R signaling plays a central role in macrophage fusion and osteoclast formation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
31,256 Da
NCBI Official Full Name
OX-2 membrane glycoprotein
NCBI Official Synonym Full Names
CD200 antigen
NCBI Official Symbol
Cd200
NCBI Official Synonym Symbols
OX2; Mox2
NCBI Protein Information
OX-2 membrane glycoprotein; MRC OX-2 antigen; antigen identified by monoclonal antibody MRC OX-2
UniProt Protein Name
OX-2 membrane glycoprotein
Protein Family
UniProt Gene Name
Cd200
UniProt Synonym Gene Names
Mox2
UniProt Entry Name
OX2G_MOUSE
Uniprot Description
CD200: Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Cellular Component: membrane; integral to membrane; plasma membrane
Molecular Function: protein binding
Research Articles on CD200
Similar Products
Product Notes
The Mouse CD200 cd200 (Catalog #AAA177168) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177168 ELISA Kit recognizes Mouse CD200. It is sometimes possible for the material contained within the vial of "CD200, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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