Rabbit anti-Mouse CD96 Polyclonal Antibody | anti-CD96 antibody

Anti-CD96 Antibody, Rabbit Polyclonal

Cat. Num. AAA8112035

• Gene Names: Cd96; Tactile; 1700109I12Rik
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Protein A & Antigen Affinity
• Synonyms: CD96; Polyclonal Antibody; Anti-CD96 Antibody; Rabbit Polyclonal; CD96 Antibody; Rabbit PAb; Antigen Affinity Purified; CD96 molecule; Anti-1700109I12Rik Antibody; Anti-Tactile Antibody; anti-CD96 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse CD96
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CD96 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000
• Immunogen: Recombinant Mouse CD96 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse CD96 (rM CD96). CD96 specific IgG was purified by Mouse CD96 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-CD96 rabbit polyclonal antibody at 1:1000 dilution Lane A: Mouse pancreas tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:67 kDa Observed band size:67 kDa)

Related Product Information for anti-CD96 antibody

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. The CD155 ligand CD96 is a member of the Ig superfamily. It's an immunoglobulin-like protein tentatively allocated to the repertoire of human NK receptors. NK cells recognize poliovirus receptor (PVR), a nectins and nectin-like protein family member serve to mediate cell-cell adhesion, cell migration, with the presence of an additional receptor, CD96. CD96 promotes NK cell adhesion to target cells expressing PVR, stimulates cytotoxicity of activated NK cells, and mediates acquisition of PVR from target cells. The effect the cells with mutated CD96 protein lost adhesion and growth activities indicates that CD96 mutations may cause a form of the C syndrome by interfering with cell adhesion and growth.

Product Categories/Family for anti-CD96 antibody

Cancer Drug Targets

References

Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12
Anja Fuchs, et al. (2004) Cutting Edge: CD96 (Tactile) Promotes NK Cell-Target Cell Adhesion by Interacting with the Poliovirus Receptor (CD155). The Journal of Immunology.172 (7): 3994-8.
Seth S, et al.(2007) The murine pan T cell marker CD96 is an adhesion receptor for CD155 and nectin-1. Biochemical and Biophysical Research Communications. 364 (4): 959-65.

NCBI and Uniprot Product Information

• NCBI GI #: 160333634
• NCBI GeneID: 84544
• NCBI Accession #: NP_115854.2
• NCBI GenBank Nucleotide #: NM_032465.2
• UniProt Accession #: Q3U0X8; Q7TMW0; Q8C9U6; Q99M67
• Molecular Weight: 67,133 Da
• NCBI Official Full Name: T-cell surface protein tactile
• NCBI Official Synonym Full Names: CD96 antigen
• NCBI Official Symbol: Cd96
• NCBI Official Synonym Symbols: Tactile; 1700109I12Rik
• NCBI Protein Information: T-cell surface protein tactile; cell surface antigen CD96; T cell activation, increased late expression; t cell-activated increased late expression protein
• UniProt Protein Name: T-cell surface protein tactile
• Protein Family: T-cell surface protein tactile
• UniProt Gene Name: Cd96
• UniProt Entry Name: TACT_MOUSE

Uniprot Description

CD96: May be involved in adhesive interactions of activated T and NK cells during the late phase of the immune response. Promotes NK cell-target adhesion by interacting with PVR present on target cells. May function at a time after T and NK cells have penetrated the endothelium using integrins and selectins, when they are actively engaging diseased cells and moving within areas of inflammation. Defects in CD96 are a cause of C syndrome (CSYN); also called Opitz trigonocephaly syndrome. This syndrome is characterized by trigonocephaly and associated anomalies, such as unusual facies, wide alveolar ridges, multiple buccal frenula, limb defects, visceral anomalies, redundant skin, psychomotor retardation and hypotonia. A chromosomal aberration involving CD96 has been found in a patient with C syndrome. Translocation t(3;18)(q13.13;q12.1). CD96 gene was located at the 3q13.13 breakpoint. Precise structural analysis around the breakpoint showed that the gene was disrupted by the translocation in exon 5, probably leading to premature termination or loss of expression of CD96 protein. No gene was detected at the chromosome 18 breakpoint. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Cellular Component: membrane; integral to membrane

Biological Process: cell adhesion