CD8 alpha recombinant protein

Recombinant CD8 alpha

Cat. Num. AAA201166

• Gene Names: CD8A; CD8; p32; Leu2
• Applications: SDS-Page
• Purity: > 95% by SDS - PAGE
• Synonyms: CD8 alpha; Recombinant CD8 alpha; CD8A; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; CD8a; MAL; CD8; Leu2; p32; T-cell surface glycoprotein CD8 alpha chain isoform 1; CD8 alpha recombinant protein

• Host: Baculovirus
• Purity/Purification: > 95% by SDS - PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.5mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 22-182aa
• Sequence: SQFRVSP LDRTWNLGET VELKCQVLLS NPTSGCSWLF QPRGAAASPT FLLYLSQNKP KAAEGLDTQR FSGKRLGDTF VLTLSDFRRE NEGYYFCSAL SNSIMYFSHF VPVFLPAKPT TTPAPRPPTP APTIASQPLS LRPEACRPAA GGAVHTRGLD FACD
• Sequence Length: 235

• Applicable Applications for CD8 alpha recombinant protein: SDS-PAGE
• Tag: His-Tag
• Predicted nterm: Ala
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
• Preparation and Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition andvisualized by coomassie blue stain)

Related Product Information for CD8 alpha recombinant protein

CD8 alpha, also known as T-cell surface glycoprotein CD8 alpha chain isoform 1, is a member of integralmembrane glycoprotein that plays an essential role in the immune response and serves multiple functions inresponses. It interacts with the T-cell receptor and the MHC class I proteins presented by antigen presentingcells. Homodimers of this protein at the cell surface provides a survival mechanism allowing conjugation and lysisof multiple target cells in NK-cells. Recombinant human CD8 alpha, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for CD8 alpha recombinant protein

Immunology

References

Harland KL., et al. (2014) Nat Commun. 5:3547.;; Chen S., et al. (2019) J Cancer. 10:697-707.

NCBI and Uniprot Product Information

• NCBI GI #: 225007536
• NCBI GeneID: 925
• NCBI Accession #: NP_001139345.1
• NCBI GenBank Nucleotide #: NP_001139345.1
• UniProt Accession #: P01732
• Molecular Weight: 18.7kDa (170aa)
• NCBI Official Full Name: T-cell surface glycoprotein CD8 alpha chain isoform 1
• NCBI Official Synonym Full Names: CD8a molecule
• NCBI Official Symbol: CD8A
• NCBI Official Synonym Symbols: CD8; p32; Leu2
• NCBI Protein Information: T-cell surface glycoprotein CD8 alpha chain
• UniProt Protein Name: T-cell surface glycoprotein CD8 alpha chain
• UniProt Gene Name: CD8A
• UniProt Synonym Gene Names: MAL
• UniProt Entry Name: CD8A_HUMAN

NCBI Description

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]