CD84 active protein

CD84 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122972

• Gene Names: Cd84; CDw84; SLAMF5; A130013D22Rik
• Purity: >97% as determined by SDS-PAGE
• Synonyms: CD84; CD84 Protein; Mouse; Recombinant (His Tag); Mouse CD84 Protein (His Tag); A130013D22Rik Protein; CDw84 Protein; SLAMF5 Protein; CD84 molecule; CD84 active protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Val221

• Species: Mouse
• Activity: Measured by its ability to bind biotinylated recombinant human SH2D1A in a functional ELISA.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Lys 22
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse CD84 isoform 1 (Q18PI6-1) extracellular domain (Met 1-Val 221) was expressed, with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CD84 active protein

Background: The CD2 family receptors are type I transmembrane glycoproteins belonging to immunoglobulin (Ig) superfamily characterized by a membrane-proximal Ig constant 2 (C2) domain and a membrane-distal variable (V) domain that is responsible for ligand recognition. CD84, also known as LY9B and SLAMF5, is a homophilic member of the SLAM (signaling lymphocyte activation molecule) subfamily of the CD2 family. The SLAM family receptorsmediate signal transduction through the interaction of its ITSM (immunoreceptor tyrosine-based switch motifs) in the intracellular region and the SH2 domain of adaptor molecules SAP (SLAM-associated protein) and EAT-2 (EWS-activated transcript 2), and accordingly modulate both adaptive and innate immune responses. The CD84-CD84 interaction was independent of its cytoplasmic tail. Thus, CD84 is its own ligand and acts as a costimulatory molecule. CD84 is expressed on cells from almost all hematopoietic lineages and on CD34+ hematopoietic progenitor cells, suggesting that CD84 serves as a marker for committed hematopoietic progenitor cells.

Product Categories/Family for CD84 active protein

ITIM/ITAM Immunoreceptors and Related Molecules

References

Martin M, et al. (2001) CD84 functions as a homophilic adhesion molecule and enhances IFN-gamma secretion: adhesion is mediated by Ig-like domain 1. J Immunol. 167(7): 3668-76.Tangye SG, et al. (2002) CD84 is up-regulated on a major population of human memory B cells and recruits the SH2 domain containing proteins SAP and EAT-2. Eur J Immunol. 32(6): 1640-9.Zaiss M, et al. (2003) CD84 expression on human hematopoietic progenitor cells. Exp Hematol. 31(9): 798-805.Tangye SG, et al. (2003) Functional requirements for interactions between CD84 and Src homology 2 domain-containing proteins and their contribution to human T cell activation. J Immunol. 171(5): 2485-95.Yan Q, et al. (2007) Structure of CD84 provides insight into SLAM family function. Proc Natl Acad Sci U S A. 104(25): 10583-8.

NCBI and Uniprot Product Information

• NCBI GI #: 357197147
• NCBI GeneID: 12523
• NCBI Accession #: NP_001239401.1
• NCBI GenBank Nucleotide #: NM_001252472.1
• UniProt Accession #: Q18PI6; Q18PI7; Q3TBT1; Q3U0A2; Q8BFV0; Q9Z178
• Molecular Weight: 37,378 Da
• NCBI Official Full Name: SLAM family member 5 isoform 2
• NCBI Official Synonym Full Names: CD84 antigen
• NCBI Official Symbol: Cd84
• NCBI Official Synonym Symbols: CDw84; SLAMF5; A130013D22Rik
• NCBI Protein Information: SLAM family member 5; leukocyte differentiation antigen CD84; signaling lymphocytic activation molecule 5
• UniProt Protein Name: SLAM family member 5
• Protein Family: SLAM family
• UniProt Gene Name: Cd84
• UniProt Synonym Gene Names: Slamf5
• UniProt Entry Name: SLAF5_MOUSE

Uniprot Description

CD84: Plays a role as adhesion receptor functioning by homophilic interactions and by clustering. Recruits SH2 domain- containing proteins SH2D1A/SAP. Increases proliferative responses of activated T-cells and SH2D1A/SAP does not seen be required for this process. Homophilic interactions enhance interferon gamma/IFNG secretion in lymphocytes and induce platelet stimulation via a SH2D1A/SAP-dependent pathway. May serve as a marker for hematopoietic progenitor cells. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell surface; Immunoglobulin superfamily

Cellular Component: membrane; integral to membrane; plasma membrane

Molecular Function: receptor activity

Biological Process: cell adhesion